Extremophiles

, Volume 18, Issue 2, pp 399–413 | Cite as

Spatial and temporal distribution of archaeal diversity in meromictic, hypersaline Ocnei Lake (Transylvanian Basin, Romania)

  • Andreea Baricz
  • Cristian Coman
  • Adrian Ştefan Andrei
  • Vasile Muntean
  • Zsolt Gyula Keresztes
  • Manuela Păuşan
  • Mircea Alexe
  • Horia Leonard Banciu
Original Paper

Abstract

Saline, meromictic lakes with significant depth are usually formed as a result of salt mining activity. Ocnei Lake is one of the largest Transylvanian (Central Romania) neutral, hypersaline lake of man-made origin. We aimed to survey the seasonal dynamics of archaeal diversity in the water column of Ocnei Lake by employing microbiological methods as well as molecular techniques based on the sequence analysis of the 16S rRNA gene. We found that archaeal diversity in the water column increased with depth and salinity, with 8 OTUs being detected in the epilimnion compared to 21 found in the chemocline, and 32 OTUs in the monimolimnion. Down to 3.5 m depth, the archaeal community was markedly dominated by the presence of an unclassified archaeon sharing 93 % sequence identity to Halogeometricum spp. At the chemocline, the shift in archaeal community composition was associated with an increase in salinity, the main factor affecting the vertical distribution of archaeal assemblages. It appears that the microoxic and hypersaline monimolimnion is populated by several major haloarchaeal taxa, with minor fluctuations in their relative abundances throughout all seasons. The culturable diversity was reasonably correlated to the dominant OTUs obtained by molecular methods. Our results indicate that Ocnei Lake represents a relatively stable extreme habitat, accommodating a diverse and putatively novel archaeal community, as 30 % of OTUs could not be classified at the genus level.

Keywords

Chemocline 16S rRNA gene clone library Epilimnion Halobacteriaceae Monimolimnion 

Supplementary material

792_2013_625_MOESM1_ESM.doc (1.2 mb)
Supplementary material 1 (DOC 1262 kb)

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Copyright information

© Springer Japan 2014

Authors and Affiliations

  • Andreea Baricz
    • 1
    • 2
  • Cristian Coman
    • 2
  • Adrian Ştefan Andrei
    • 2
    • 3
  • Vasile Muntean
    • 1
  • Zsolt Gyula Keresztes
    • 4
  • Manuela Păuşan
    • 1
  • Mircea Alexe
    • 5
  • Horia Leonard Banciu
    • 1
    • 3
  1. 1.Department of Molecular Biology and Biotechnology, Faculty of Biology and GeologyBabeş-Bolyai UniversityCluj-NapocaRomania
  2. 2.National Institute of Research and Development for Biological Sciences (NIRDBS)Institute of Biological ResearchCluj-NapocaRomania
  3. 3.Institute for Interdisciplinary Research in Bio-Nano-SciencesBabeş-Bolyai UniversityCluj-NapocaRomania
  4. 4.Balaton Limnological Institute, Centre for Ecological ResearchHungarian Academy of SciencesTihanyHungary
  5. 5.Faculty of GeographyBabeş-Bolyai UniversityCluj-NapocaRomania

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