Extremophiles

, Volume 18, Issue 1, pp 121–130

Isolation and characterization of phage–host systems from the Baltic Sea ice

  • Anne-Mari Luhtanen
  • Eeva Eronen-Rasimus
  • Hermanni Kaartokallio
  • Janne-Markus Rintala
  • Riitta Autio
  • Elina Roine
Original Paper

DOI: 10.1007/s00792-013-0604-y

Cite this article as:
Luhtanen, AM., Eronen-Rasimus, E., Kaartokallio, H. et al. Extremophiles (2014) 18: 121. doi:10.1007/s00792-013-0604-y

Abstract

In search for sea ice bacteria and their phages from the Baltic Sea ice, two ice samples were collected from land-fast ice in a south-west Finland coastal site in February and March 2011. Bacteria were isolated from the melted sea ice samples and phages were screened from the same samples for 43 purified isolates. Plaque-producing phages were found for 15 bacterial isolates at 3 °C. Ten phage isolates were successfully plaque purified and eight of them were chosen for particle purification to analyze their morphology and structural proteins. Phage 1/32 infecting an isolate affiliated to phylum Bacteroidetes (Flavobacterium sp.) is a siphovirus and six phages infecting isolates affiliated to γ-Proteobacteria (Shewanella sp.) hosts were myoviruses. Cross titrations between the hosts showed that all studied phages are host specific. Phage solutions, host growth and phage infection were tested in different temperatures revealing phage temperature tolerance up to 45 °C, whereas phage infection was in most of the cases retarded above 15 °C. This study is the first to report isolation and cultivation of ice bacteria and cold-active phages from the Baltic Sea ice.

Keywords

Baltic Sea Sea ice Flavobacterium Shewanella Phage Cold-active 

Abbreviations

BSA

Bovine serum albumin

DGGE

Denaturing gradient gel electrophoresis

DOM

Dissolved organic matter

dsDNA

Double-stranded DNA

PEG

Polyethylene glycol

Pfu

Plaque forming units

PVDF

Polyvinylidene difluoride

SW

South-west

TEM

Transmission electron microscopy

TGGE

Temperature gradient gel electrophoresis

Copyright information

© Springer Japan 2013

Authors and Affiliations

  • Anne-Mari Luhtanen
    • 1
    • 2
    • 3
  • Eeva Eronen-Rasimus
    • 2
    • 4
  • Hermanni Kaartokallio
    • 2
  • Janne-Markus Rintala
    • 2
    • 3
    • 4
  • Riitta Autio
    • 2
  • Elina Roine
    • 1
  1. 1.Department of BiosciencesUniversity of HelsinkiHelsinkiFinland
  2. 2.Finnish Environment InstituteMarine Research CentreHelsinkiFinland
  3. 3.Tvärminne Zoological StationUniversity of HelsinkiHankoFinland
  4. 4.Department of Environmental SciencesUniversity of HelsinkiHelsinkiFinland

Personalised recommendations