, Volume 17, Issue 6, pp 981–993 | Cite as

Production of biosurfactant on crude date syrup under saline conditions by entrapped cells of Natrialba sp. strain E21, an extremely halophilic bacterium isolated from a solar saltern (Ain Salah, Algeria)

  • Salima Kebbouche-GanaEmail author
  • Mohamed Lamine Gana
  • Imen Ferrioune
  • Souad Khemili
  • Nesrine Lenchi
  • Sihem Akmouci-Toumi
  • Nabila Amel Bouanane-Darenfed
  • Nacer-Eddine Djelali
Original Paper


A bacterial strain E21 was isolated from a sample of water collected in the salt lake located close to Ain Salah, Algeria. The analysis of 16S rRNA gene sequence had indicated that the strain had 93 % sequence similarity with the genus Natrialba sp. strain E21 (GenBank, FR750525.1) and was considered extremely halophilic. Production of biosurfactant by the strain E21 with free and entrapped cells was investigated using soluble starch in the saline conditions. Biosurfactant synthesis was followed by measuring the surface tension and emulsifying index 9 days under optimal conditions (40 °C, pH 7). Some diffusional limitations in alginate and agar beads affected the kinetics of biosurfactant production when compared to that obtained with free cells culture. The minimum values of surface tension were 27 and 30 mN m−1 achieved after 9 days with free and immobilized cells, respectively, while the corresponding maximum E24 values were 65.3 and 62.3 %, respectively. The re-use of bacterial cells along with the limited cell losses provided by the immobilized system might lead to significant reduction of the biosurfactant production cost.


Natrialba sp. strain E21 Cells immobilization Alginate Biosurfactant production 



This work was supported by the assistance of the Algerian Ministry of Education grant PNR project (14/u35/1360)


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Copyright information

© Springer Japan 2013

Authors and Affiliations

  • Salima Kebbouche-Gana
    • 1
    Email author
  • Mohamed Lamine Gana
    • 2
  • Imen Ferrioune
    • 1
  • Souad Khemili
    • 1
  • Nesrine Lenchi
    • 1
  • Sihem Akmouci-Toumi
    • 1
  • Nabila Amel Bouanane-Darenfed
    • 3
  • Nacer-Eddine Djelali
    • 4
  1. 1.Laboratoire Conservation et Valorisation des Ressources Biologiques, Faculté des SciencesUniversité M’Hamed Bougara de BoumerdesBoumerdèsAlgeria
  2. 2.Centre de Recherche et de DéveloppementSONATRACHBoumerdèsAlgeria
  3. 3.Laboratoire de Microbiologie, Faculté des Sciences BiologiquesU.S.T.H.BAlgerAlgeria
  4. 4.Faculté des SciencesUniversité M’Hamed Bougara de BoumerdesBoumerdèsAlgeria

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