Extremophiles

, Volume 17, Issue 6, pp 897–909

l-Arabinose degradation pathway in the haloarchaeon Haloferax volcanii involves a novel type of l-arabinose dehydrogenase

  • Ulrike Johnsen
  • Jan-Moritz Sutter
  • Henning Zaiß
  • Peter Schönheit
Original Paper

Abstract

The pathway of l-arabinose degradation was studied in the haloarchaeon Haloferax volcanii. It is shown that l-arabinose is oxidatively degraded to α-ketoglutarate. During growth on l-arabinose, l-arabinose dehydrogenase (l-AraDH) was induced. The enzyme was purified as a 130 kDa homotetrameric protein catalyzing the oxidation of l-arabinose with both NADP+ and NAD+. The gene encoding l-AraDH was identified as HVO_B0032 and recombinant l-AraDH showed similar properties as the native enzyme. The l-AraDH deletion mutant did not grow on l-arabinose, but grew unaffected on glucose and d-xylose, indicating a specific involvement in l-arabinose degradation. Phylogenetic analyses attribute the first archaeal l-AraDH to the extended short-chain dehydrogenase/reductase (SDRe) family, where it is part of a novel cluster and thus differs from known archaeal and bacterial pentose dehydrogenases. Further, cell extracts of H. volcanii catalyzed the NADP+-dependent conversion of l-arabinoate to α-ketoglutarate. The genes involved in that conversion were identified by analyses of transcripts and deletion mutants as HVO_B0038A, HVO_B0027 and HVO_B0039 recently reported to be involved in d-xylonate conversion to α-ketoglutarate in H. volcanii (Johnsen et al. 2009).

Keywords

l-Arabinose dehydrogenase Archaea Haloferax volcanii Metabolic pathways Extended short-chain dehydrogenase/reductase Pentose catabolism 

Supplementary material

792_2013_572_MOESM1_ESM.docx (440 kb)
Supplementary material (DOCX 440 kb)

Copyright information

© Springer Japan 2013

Authors and Affiliations

  • Ulrike Johnsen
    • 1
  • Jan-Moritz Sutter
    • 1
  • Henning Zaiß
    • 1
  • Peter Schönheit
    • 1
  1. 1.Institut für Allgemeine MikrobiologieChristian-Albrechts-Universität KielKielGermany

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