Extremophiles

, Volume 17, Issue 5, pp 809–819 | Cite as

Characterization of a cold-adapted and salt-tolerant esterase from a psychrotrophic bacterium Psychrobacterpacificensis

  • Guojie Wu
  • Gaobing Wu
  • Tao Zhan
  • Zongze Shao
  • Ziduo Liu
Original Paper

Abstract

An esterase gene, est10, was identified from the genomic library of a deep-sea psychrotrophic bacterium Psychrobacterpacificensis. The esterase exhibited the optimal activity around 25 °C and pH 7.5, and maintained as high as 55.0 % of its maximum activity at 0 °C, indicating its cold adaptation. Est10 was fairly stable under room temperatures, retaining more than 80 % of its original activity after incubation at 40 °C for 2 h. The highest activity was observed against the short-chain substrate p-nitrophenyl butyrate (C4) among the tested p-nitrophenyl esters (C2–C16). It was slightly activated at a low concentration (1 mM) of Zn2+, Mg2+, Ba2+, Ca2+, Cu2+, Fe3+, urea and EDTA, but was inhibited by DTT and totally inactivated by PMSF. Interestingly, increased salinity considerably stimulated Est10 activity (up to 143.2 % of original activity at 2 M NaCl) and stability (up to 126.4 % after incubation with 5 M NaCl for 6.5 h), proving its salt tolerance. 0.05 and 0.1 % Tween 20, Tween 80, Triton X-100 and CHAPS increased the activity and stability of Est10 while SDS, CTAB had the opposite effect. Est10 was quite active after incubation with several 30 % organic solvents (methanol, DMSO, ethanediol) but exhibited little activity with 30 % isopropanol, ethanol, n-butanol and acetonitrile.

Keywords

Esterase Psychrotrophic bacterium Cold adaptation Salt tolerance Stability 

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Copyright information

© Springer Japan 2013

Authors and Affiliations

  • Guojie Wu
    • 1
  • Gaobing Wu
    • 2
  • Tao Zhan
    • 1
  • Zongze Shao
    • 3
  • Ziduo Liu
    • 1
  1. 1.State Key Laboratory of Agricultural Microbiology, College of Life Science and TechnologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  3. 3.Key Laboratory of Marine Biogenetic Resources, The Third Institute of OceanographyState of Oceanic AdministrationXiamenPeople’s Republic of China

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