Isolation and characterization of two novel alkalitolerant sulfidogens from a Thiopaq bioreactor, Desulfonatronum alkalitolerans sp. nov., and Sulfurospirillum alkalitolerans sp. nov
- 730 Downloads
Two obligately anaerobic sulfidogenic bacterial strains were isolated from the full-scale Thiopaq bioreactor in Lelystad (The Netherlands) removing H2S from biogas under oxygen-limiting and moderately haloalkaline conditions. Strain HSRB-L represents a dominant culturable sulfate-reducing bacterium in the reactor. It utilizes formate, H2 (with acetate as C-source) and lactate as e-donors, and sulfate, thiosulfate and sulfite as e-acceptors. It is haloalkalitolerant, with a pH range for lithotrophic growth from 7.5 to 9.7 (optimum at 8.5–9) and a salt range from 0.1 to 1.75 M total Na+ (optimum at 0.6 M). The strain is a member of the genus Desulfonatronum and is proposed as a novel species D. alkalitolerans. The second strain, strain HTRB-L1, represents a dominant thiosulfate/sulfur reducer in the reactor. It is an obligate anaerobe utilizing formate and H2 (with acetate as C-source), lactate, pyruvate and fumarate as e-donors, and thiosulfate (incomplete reduction), sulfur, arsenate and fumarate as e-acceptors. With lactate as e-donor it also grows as an ammonifyer in the presence of nitrate and nitrite. HTRB-L1 is haloalkalitolerant, with a pH range for lithotrophic growth from 7.1 to 9.7 (optimum at 8.5) and a salt range from 0.6 to 1.5 M total Na+ (optimum at 0.6 M). Phylogenetic analysis showed that strain HTRB-L1 is a novel species within the genus Sulfurospirillum (Epsilonproteobacteria) for which a name Sulfurospirillum alkalitolerans is proposed.
KeywordsThiopaq bioreactor Sulfur-reducing Thiosulfate-reducing Alkalitolerant
This work was supported by the RFBR Grant 13-04-00049 to DS, and by an Advanced ERC Grant to GM. We are grateful to Erik van Zessen for providing samples from the Thiopaq bioreactor.
- Gries-Romijn van Eck (1966) Physiological and chemical test for drinking water. NEN 1056, IY-2 Nederlandse Normalisatie Instituut RijswijkGoogle Scholar
- Janssen AJH, Lettinga G, de Keizer A (1999) Removal of hydrogen sulfide from wastewater and waste gases by biological conversion to elemental sulfur. Colloidal and interfacial aspects of biologically produced sulfur particles. Colloids Surf A Physicochem Eng Asp 151:389–397Google Scholar
- Luijten MLGC, de Weert J, Smidt H, Boschker HTS, de Vos WM, Schraa G, Stams AJM (2003) Description of Sulfurospirillum halorespirans sp. nov., an anaerobic, tetrachloroethene- respiring bacterium, and transfer of Dehalospirillum multivorans to the genus Sulfurospirillum as Sulfurospirillum multivorans comb. nov. Int J Syst Evol Microbiol 53:787–793PubMedCrossRefGoogle Scholar
- Pikuta EV, Zhilina TN, Zavarzin GA, Kostrikina NA, Osipov GA, Rainey FA (1998) Desulfonatronum lacustre gen. nov., sp. nov.: a new alkaliphilic sulfate-reducing bacterium utilizing ethanol. Microbiology (Moscow, English translation) 67:105–113Google Scholar
- Schäfer H, Muyzer G (2001) Denaturing gradient gel electrophoresis in marine microbial ecology. In: Paul JH (ed) Methods in microbiology. Academic, New York, pp 425–468Google Scholar
- Simon J, Kroneck PMH (2013) Microbial sulfite respiration. Adv Microb Physiol 62:45–117Google Scholar
- Sorokin DY, Kuenen JG, Muyzer G (2011a) The microbial sulfur cycle in soda lakes. Front Microbial Physiol 2, article 44Google Scholar
- Sorokin DY, Tourova TP, Detkova EN, Kolganova TV, Galinski EA, Muyzer G (2011b) Culturable diversity of lithotrophic haloalkaliphilic sulfate-reducing bacteria in soda lakes and the description of Desulfonatronum thioautotrophicum sp. nov., Desulfonatronum thiosulfatophilum sp. nov., Desulfonatronovibrio thiodismutans sp. nov., and Desulfonatronovibrio magnus sp. nov. Extremophiles 15:391–401PubMedCrossRefGoogle Scholar
- Sorokin DY, Panteleeva AN, Muntyan MS, Muyzer G (2012) Thioalkalivibrio sulfidophilus sp. nov., a haloalkaliphilic sulfur-oxidizing gammaproteobacterium from alkaline habitats. Int J Syst Evol Microbiol 62:1884–1889Google Scholar