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Extremophiles

, Volume 17, Issue 3, pp 499–504 | Cite as

Haloferax chudinovii sp. nov., a halophilic archaeon from Permian potassium salt deposits

  • Alexander I. Saralov
  • Roman V. Baslerov
  • Boris B. Kuznetsov
Original Paper

Abstract

Three pigmented strains of halophilic archaea (RS75, RS77, RS79) were isolated from the monoliths of mottled sylvinite from the Verkhnekamsk salt deposit (Solikamsk, Russia). The cells were nonmotile, gram-negative, pleomorphic, disk-shaped or ovoid, 0.8–1.0 × 1.5–2.5 μm. The organism was a chemoorganotrophic obligate aerobe producing catalase and oxidase. A number of carbohydrates and carboxylic acids were used as growth substrates. Growth occurred in the presence of 7–27 % NaCl (with the optimum at 15–18 %), 0.02–20 % KCl (0.2–1 %), 0.2–16 % MgCl2 (2–3 %), in the temperature range from 23 to 51 °C (40–45 °C), and pH 5.5–8.0 (6.8–7.0). The membranes contained carotenoids of the bacterioruberin series. Phosphatidylglyceromethylphosphate (PGP-Me), phosphatidylglycerol (PG), sulfated diglycosyl diether (S-DGD-1) predominated among the polar lipids. The DNA G + C content was 64.0–65.0 mol %. Phylogenetic analysis of the 16S rRNA gene sequences showed high similarity of the new strains to Haloferax species: H. denitrificans (99.2 %) and H. volcanii (99.1 %), H. larsenii (96.9 %) and H. elongans (96.6 %). DNA–DNA hybridization revealed 93–95 % similarity between strain RS75 and strains RS77 and RS79; the similarity levels between strain RS75 and the type strains of Haloferax denitrificans VKM B-1754T and Halobacterium salinarum VKM B-1769T were 50 and 10 %, respectively. According to its phenotypic and genotypic characteristics, the organism was classified as a member of the genus Haloferax, forming a new species with the proposed name Haloferax chudinovii sp. nov. type strain is RS75T (=VKPM B-11279T).

Keywords

Haloarchaea Haloferax Haloferax chudinovii Permian 

Notes

Acknowledgments

This work was supported by the program no. 22 (Molecular and Cell Biology) of the Presidium of the Russian Academy of Sciences, project no. 01200963684, and by the state contract of the Russian Ministry of Education and Science no. 16.552.11.7035.

Supplementary material

792_2013_534_MOESM1_ESM.doc (1.1 mb)
Supplementary material 1 (DOC 1083 kb)
792_2013_534_MOESM2_ESM.doc (139 kb)
Supplementary material 2 (DOC 139 kb)

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Copyright information

© Springer Japan 2013

Authors and Affiliations

  • Alexander I. Saralov
    • 1
  • Roman V. Baslerov
    • 2
  • Boris B. Kuznetsov
    • 2
  1. 1.Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of SciencesPermRussia
  2. 2.Center Bioengineering, Russian Academy of SciencesMoscowRussia

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