, Volume 17, Issue 3, pp 471–476 | Cite as

Isoptericola salitolerans sp. nov., a halotolerant filamentous actinobacterium isolated from a salt lake, China

  • Tong-Wei Guan
  • Yun Teng
  • Ling-Ling Yang
  • Xiao-Ping Zhang
  • Zhen-Ming Che
Original Paper


A novel bacterial strain, TRM F109T, was isolated from hypersaline habitat in Sichuan Province, China. It was a Gram-positive, aerobic, non-motile, halotolerant, filamentous bacterium. Phylogenetic analysis based on 16S rRNA gene sequences revealed levels of similarity of 97.0–98.4 % to the type strains of recognized species of the genus Isoptericola. Chemotaxonomic data also supported the placement of strain TRM F109T within the genus Isoptericola. The low levels of DNA–DNA relatedness between the novel strain and the type strains of recognized species of the genus Isoptericola, in combination with differential phenotypic data, demonstrate that strain TRM F109T represents a novel species of the genus Isoptericola, for which the name Isoptericola salitolerans sp. nov. is proposed. The type strain is TRM F109T (=JCM 15901T=KCTC 19617T).


Isoptericola salitolerans sp. nov. Polyphasic taxonomy Daying salt lake 



This research was supported by National Natural Science Foundation of China (Project No. 31060003), Key Research Foundation of Xihua University (Z1220530), Key Project of Office of Education of Sichuan Province, China (Project No. 2013) and Key Project for Department of Science and Technology of Ministry of Education of China (Project No. 209145).

Supplementary material

792_2013_531_MOESM1_ESM.pdf (84 kb)
Supplementary material 1 (PDF 83 kb)


  1. Bakalidou A, Kämpfer P, Berchtold M, Kuhnigk T, Wenzel M, König H (2002) Cellulosimicrobium variabile sp. nov., a cellulolytic bacterium from the hindgut of the termite Mastotermes darwiniensis. Int J Syst Evol Microbiol 5:1185–1192CrossRefGoogle Scholar
  2. Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230PubMedCrossRefGoogle Scholar
  3. Cui XL, Mao PH, Tseng M, Li WJ, Zhang LP, Xu LH, Jiang CL (2001) Streptomonospora salina gen. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51:357–363PubMedGoogle Scholar
  4. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376PubMedCrossRefGoogle Scholar
  5. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789CrossRefGoogle Scholar
  6. Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416CrossRefGoogle Scholar
  7. Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) (1994) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DCGoogle Scholar
  8. Gordon RE, Barnett DA, Handerhan JE, Pang CHN (1974) Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63CrossRefGoogle Scholar
  9. Groth I, Schumann P, Rainey FA, Martin K, Schuetze B, Augsten K (1997) Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133PubMedCrossRefGoogle Scholar
  10. Groth I, Schumann P, Schütze B, Gonzalez JM, Laiz L, Cesareo SJ, Stackebrandt E (2005) Isoptericola hypogeus sp. nov., isolated from the Roman catacomb of Domitilla. Int J Syst Evol Microbiol 55:1715–1719PubMedCrossRefGoogle Scholar
  11. Guan TW, Zhao K, Xiao J, Liu Y, Xia ZF, Zhang XP, Zhang LL (2010) Brevibacterium salitolerans sp. nov., an actinobacterium isolated from a salt lake in Xinjiang, China. Int J Syst Evol Microbiol 60:2991–2995PubMedCrossRefGoogle Scholar
  12. He L, Li W, Huang Y, Wang L, Liu Z, Lanoot B, Vancanneyt M, Swings J (2005) Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55:1939–1944PubMedCrossRefGoogle Scholar
  13. Huang Z, Sheng XF, Zhao F, He LY, Huang J, Wang Q (2012) Isoptericola nanjingensis sp. nov., a mineral-weathering bacterium. Int J Syst Evol Microbiol 62:971–976PubMedCrossRefGoogle Scholar
  14. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16:111–120PubMedCrossRefGoogle Scholar
  15. Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R, Xu LH, Stackebrandt E, Jiang CL (2007) Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57:1424–1428PubMedCrossRefGoogle Scholar
  16. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167CrossRefGoogle Scholar
  17. Minnikin DE, Hutchinson IG, Caldicott AB, Goodfellow M (1980) Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233CrossRefGoogle Scholar
  18. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241CrossRefGoogle Scholar
  19. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol Biol Evol 4:406–425PubMedGoogle Scholar
  20. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. IDI Inc., NewarkGoogle Scholar
  21. Schleifer KH, Kandler O (1972) Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477PubMedGoogle Scholar
  22. Stackebrandt E, Rainey FA, Ward-Rainey NL (1997) Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491CrossRefGoogle Scholar
  23. Stackebrandt E, Schumann P, Cui XL (2004) Reclassification of Cellulosimicrobium variabile Bakalidou et al. 2002 as Isoptericola variabilis gen. nov., comb. nov. Int J Syst Evol Microbiol 54:685–688PubMedCrossRefGoogle Scholar
  24. Staneck JL, Roberts GD (1974) Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol 28:226–231PubMedGoogle Scholar
  25. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739PubMedCrossRefGoogle Scholar
  26. Tang SK, Wang Y, Chen Y, Lou K, Cao LL, Xu LH, Li WJ (2009) Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. Int J Syst Evol Microbiol 59:2025–2032PubMedCrossRefGoogle Scholar
  27. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882PubMedCrossRefGoogle Scholar
  28. Tseng M, Liao HC, Chiang WP, Yuan GF (2011) Isoptericola chiayiensis sp. nov., isolated from mangrove soil. Int J Syst Evol Microbiol 61:1667–1670PubMedCrossRefGoogle Scholar
  29. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE (1987) International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464CrossRefGoogle Scholar
  30. Wu Y, Li WJ, Tian W, Zhang LP, Xu L, Shen Q, Shen B (2010) Isoptericola jiangsuensis sp. nov., a chitin-degrading bacterium. Int J Syst Evol Microbiol 60:904–908PubMedCrossRefGoogle Scholar
  31. Yoon JH, Schumann P, Kang SJ, Jung SY, Oh TK (2006) Isoptericola dokdonensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:2893–2897PubMedCrossRefGoogle Scholar
  32. Zhang YQ, Schumann P, Li WJ, Chen GZ, Tian XP, Stackebrandt E, Xu LH, Jiang CL (2005) Isoptericola halotolerans sp. nov., a novel actinobacterium isolated from saline soil from Qinghai Province, north-west China. Int J Syst Evol Microbiol 55:1867–1870PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2013

Authors and Affiliations

  • Tong-Wei Guan
    • 1
  • Yun Teng
    • 2
  • Ling-Ling Yang
    • 3
  • Xiao-Ping Zhang
    • 2
  • Zhen-Ming Che
    • 1
  1. 1.School of BioengineeringXihua UniversityChengduPeople’s Republic of China
  2. 2.Faculty of Resource and Environmental SciencesSichuan Agricultural UniversityYaanPeople’s Republic of China
  3. 3.The Key Laboratory for Microbial Resources of the Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of MicrobiologyYunnan UniversityKunmingPeople’s Republic of China

Personalised recommendations