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Extremophiles

, Volume 17, Issue 2, pp 251–263 | Cite as

Thermodesulfobacterium geofontis sp. nov., a hyperthermophilic, sulfate-reducing bacterium isolated from Obsidian Pool, Yellowstone National Park

  • Scott D. Hamilton-Brehm
  • Robert A. Gibson
  • Stefan J. Green
  • Ellen C. Hopmans
  • Stefan Schouten
  • Marcel T. J. van der Meer
  • John P. Shields
  • Jaap S. S. Damsté
  • James G. ElkinsEmail author
Original Paper

Abstract

A novel sulfate-reducing bacterium designated OPF15T was isolated from Obsidian Pool, Yellowstone National Park, Wyoming. The phylogeny of 16S rRNA and functional genes (dsrAB) placed the organism within the family Thermodesulfobacteriaceae. The organism displayed hyperthermophilic temperature requirements for growth with a range of 70–90 °C and an optimum of 83 °C. Optimal pH was around 6.5–7.0 and the organism required the presence of H2 or formate as an electron donor and CO2 as a carbon source. Electron acceptors supporting growth included sulfate, thiosulfate, and elemental sulfur. Lactate, acetate, pyruvate, benzoate, oleic acid, and ethanol did not serve as electron donors. Membrane lipid analysis revealed diacyl glycerols and acyl/ether glycerols which ranged from C14:0 to C20:0. Alkyl chains present in acyl/ether and diether glycerol lipids ranged from C16:0 to C18:0. Straight, iso- and anteiso-configurations were found for all lipid types. The presence of OPF15T was also shown to increase cellulose consumption during co-cultivation with Caldicellulosiruptor obsidiansis, a fermentative, cellulolytic extreme thermophile isolated from the same environment. On the basis of phylogenetic, phenotypic, and structural analyses, Thermodesulfobacterium geofontis sp. nov. is proposed as a new species with OPF15T representing the type strain.

Keywords

Dissimilatory sulfate reduction Hyperthermophile Thermal environments Thermodesulfobacterium Membrane lipids 

Abbreviations

DSR

Dissimilatory sulfite reductase

SRM

Sulfate-reducing microorganisms

YNP

Yellowstone National Park

OP

Obsidian Pool

IPLs

Intact polar lipids

PE

Phosphoethanolamine

APT

Phosphoaminopentanetetrol

Phex

Phosphohexose

DAG

Diacyl glycerol

AEG

Acyl/ether glycerol

DEG

Diether glycerol

Notes

Acknowledgments

We thank Sarah J. Kauffman for her invaluable technical assistance. We also thank Brian P. Hedlund, Christie Hendrix, and the National Park Service for sample collection under permit #YELL-SCI-0115 (PI: Karl O. Stetter). Christopher W. Schadt and Dwayne A. Elias provided helpful comments during preparation of the manuscript. Support for S. D. H.-B. and J. G. E. was provided by the BioEnergy Science Center (BESC), which is a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science, Oak Ridge National Laboratory. Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the U.S. Department of Energy under contract DE-AC05-00OR22725. The research was partially funded by the Netherlands Darwin Centre for Biogeosciences by a grant for a post-doctoral fellowship (R.A.G.) to J.S.S.D.

Supplementary material

792_2013_512_MOESM1_ESM.pptx (56 kb)
Supplementary material 1 (PPTX 56 kb)

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Copyright information

© Springer Japan (outside the USA)  2013

Authors and Affiliations

  • Scott D. Hamilton-Brehm
    • 1
  • Robert A. Gibson
    • 2
  • Stefan J. Green
    • 3
  • Ellen C. Hopmans
    • 2
  • Stefan Schouten
    • 2
  • Marcel T. J. van der Meer
    • 2
  • John P. Shields
    • 4
  • Jaap S. S. Damsté
    • 2
  • James G. Elkins
    • 1
    Email author
  1. 1.Biosciences Division, Oak Ridge National LaboratoryBioEnergy Science CenterOak RidgeUSA
  2. 2.Department of Marine Organic BiogeochemistryNIOZ Royal Netherlands Institute for Sea ResearchTexelThe Netherlands
  3. 3.DNA Services Facility, Research Resource CenterUniversity of Illinois at ChicagoChicagoUSA
  4. 4.Center for Advanced Ultrastructural ResearchThe University of GeorgiaAthensUSA

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