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Extremophiles

, Volume 17, Issue 1, pp 87–98 | Cite as

Phylogenetic diversity of bacterial and archaeal communities inhabiting the saline Lake Red located in Sovata, Romania

  • Andrea K. BorsodiEmail author
  • Tamás Felföldi
  • István Máthé
  • Vivien Bognár
  • Mónika Knáb
  • Gergely Krett
  • Laura Jurecska
  • Erika M. Tóth
  • Károly Márialigeti
Original Paper

Abstract

Lake Red is one of the saline lakes which were formed as a consequence of salt massif dissolution at the foot of the Gurghiu Mountains (Central Romania) at the end of the nineteenth century. The lake water had approximately 15 % w/v salt content. Phylogenetic diversity of prokaryotes inhabiting the water and sediment of the lake was studied using cultivation and cultivation-independent methods following a sampling in spring 2009. According to the results of 16S rRNA gene-based denaturing gradient gel electrophoresis (DGGE), the richness of Bacteria was higher than Archaea on the basis of the number and position of dominant bands in the gel. Sequences from DGGE bands were affiliated with Gammaproteobacteria (Halomonas and Alkalilimnicola) and Bacteroidetes (Psychroflexus) as well as Euryarchaeota. Cultivation from five different saline media resulted in 101 bacterial strains of which Gammaproteobacteria (Halomonas, Marinobacter and Salinivibrio) were the most abundant. Firmicutes (Bacillus) and Alphaproteobacteria (Aurantimonas and Roseovarius) were also identified among the isolated strains. The 16S rRNA genes from 82 bacterial and 95 archaeal clones were also phylogenetically analyzed. Bacterial clones were related to various genera of Gammaproteobacteria (Alkalilimnicola, Alkalispirillum, Arhodomonas, Halomonas, Saccharospirillum), Bacteroidetes (Gracilimonas, Psychroflexus) and Alphaproteobacteria (Oceanicola, Roseinatronobacter, Roseovarius). All of the archaeal clones sequenced corresponded to a homologous cluster affiliated with Halopelagius.

Keywords

Saline lake Phylogenetic diversity 16S rRNA gene Cultivation Denaturing gradient gel electrophoresis Cloning 

Notes

Acknowledgments

The technical contribution of Gábor Cebe and Balázs Vajna is gratefully acknowledged.

Supplementary material

792_2012_496_MOESM1_ESM.docx (15 kb)
Supplementary Table 1 PCR primer sequences and thermal profiles used for the amplification of 16S rRNA gene fragments from Bacteria and Archaea (DOCX 14 kb)
792_2012_496_MOESM2_ESM.docx (46 kb)
Supplementary Fig. 1 Rarefaction curves for the different ARDRA patterns of 16S rRNA gene bacterial and archaeal clones retrieved from Lake Red (DOCX 45 kb)
792_2012_496_MOESM3_ESM.jpg (164 kb)
Supplementary Fig. 2 Distribution of Lake Red Strains and clones within the bacterial genera (JPEG 163 kb)

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Copyright information

© Springer Japan 2012

Authors and Affiliations

  • Andrea K. Borsodi
    • 1
    Email author
  • Tamás Felföldi
    • 1
  • István Máthé
    • 2
  • Vivien Bognár
    • 1
  • Mónika Knáb
    • 1
  • Gergely Krett
    • 1
  • Laura Jurecska
    • 3
  • Erika M. Tóth
    • 1
  • Károly Márialigeti
    • 1
  1. 1.Department of MicrobiologyEötvös Loránd UniversityBudapestHungary
  2. 2.Department of BioengineeringSapientia Hungarian University of TransylvaniaMiercurea CiucRomania
  3. 3.Cooperative Research Center for Environmental SciencesEötvös Loránd UniversityBudapestHungary

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