, Volume 16, Issue 6, pp 895–901 | Cite as

Halohasta litorea gen. nov. sp. nov., and Halohasta litchfieldiae sp. nov., isolated from the Daliang aquaculture farm, China and from Deep Lake, Antarctica, respectively

Original Paper


Two halophilic archaeal strains, R30T and tADLT, were isolated from an aquaculture farm in Dailing, China, and from Deep Lake, Antarctica, respectively. Both have rod-shaped cells that lyse in distilled water, stain Gram-negative and form red-pigmented colonies. They are neutrophilic, require >120 g/l NaCl and 48–67 g/l MgCl2 for growth but differ in their optimum growth temperatures (30 °C, tADLT vs. 40 °C, R30T). The major polar lipids were typical for members of the Archaea but also included a major glycolipid chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1). The 16S rRNA gene sequences of the two strains are 97.4 % identical, show most similarity to genes of the family Halobacteriaceae, and cluster together as a distinct clade in phylogenetic tree reconstructions. The rpoB′ gene similarity between strains R30T and tADLT is 92.9 % and less to other halobacteria. Their DNA G + C contents are 62.4–62.9 mol % but DNA–DNA hybridization gives a relatedness of only 44 %. Based on phenotypic, chemotaxonomic and phylogenetic properties, we describe two new species of a novel genus, represented by strain R30T (= CGMCC 1.10593T = JCM 17270T) and strain tADLT (= JCM 15066T = DSMZ 22187T) for which we propose the names Halohasta litorea gen. nov., sp. nov. and Halohasta litchfieldiae sp. nov., respectively.


Halohasta litorea gen. nov., sp. nov. Halohasta litchfieldiae sp. nov. Halophilic archaea Deep Lake Marine solar saltern 



This work was supported by the National Natural Science Foundation of China (No. 30970006), the opening project of State key Laboratory of Microbial Resources (No. SKLMR-20100604, Institute of Microbiology, Chinese Academy of Sciences) and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). Deep Lake water samples were kindly supplied by J. Hoffman (J. Craig Venter Inst., USA) and R. Cavicchioli (University of NSW, Sydney, Australia). M. D.-S. is grateful to D. Oesterhelt (Max-Planck Institute, Munich, Germany) for providing laboratory resources for part of this study. We thank Jean Euzéby (École National Vétérinaire, Toulouse, France) for his suggestion and advice regarding the formulation of genus and species names.

Supplementary material

792_2012_485_MOESM1_ESM.doc (2.5 mb)
Supplementary material 1 (DOC 2598 kb)


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Copyright information

© Springer Japan 2012

Authors and Affiliations

  1. 1.School of Food and Biological EngineeringJiangsu UniversityZhenjiangPeople’s Republic of China
  2. 2.AdelaideAustralia
  3. 3.School of Biomedical SciencesCharles Sturt UniversityWagga WaggaAustralia

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