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Extremophiles

, Volume 16, Issue 6, pp 863–870 | Cite as

Microscopic studies on Thermosipho globiformans implicate a role of the large periplasm of Thermotogales

  • Tomohiko KuwabaraEmail author
  • Kensuke Igarashi
Original Paper

Abstract

Thermosipho globiformans is a member of Thermotogales, which contains rod-shaped, Gram-negative, anaerobic (hyper)thermophiles. These bacteria are characterized by an outer sheath-like envelope, the toga, which includes the outer membrane and an amorphous layer, and forms large periplasm at the poles of each rod. The cytoplasmic membrane and its contents are called “cell”, and the toga and its contents “rod”, to distinguish between them. Optical cells were constructed to observe binary fission of T. globiformans. High-temperature microscopy of rods adhering to optical cells' coverslips showed that the large periplasm forms between newly divided cells in a rod, followed by rod fission at the middle of the periplasm, which was accompanied by a sideward motion of the newly generated rod pole(s). Electron microscopic observations revealed that sessile rods grown on a glass plate have nanotubes adhered to the glass, and these may be involved in the sideward motion. Epifluorescence microscopy with a membrane-staining dye suggested that formation of the septal outer membrane is distinct from cytokinesis. Transmission electron microscopy indicated that the amorphous layer forms in the periplasm between already-divided cells. These findings suggest that the large periplasm is the structure in which the septal toga forms, an event separate from cytokinesis.

Keywords

Anaerobic bacteria Cell division Deep-sea thermophiles Nanotubes Toga 

Abbreviations

ATOC

Anaerobic thermophile observation chamber

FE-SEM

Field-emission scanning electron microscopy

HTM

High-temperature microscopy

OM

Outer membrane

TEM

Transmission electron microscopy

Notes

Acknowledgments

We thank Drs. Shigeru Deguchi and Sada-atsu Mukai of the Japan Agency for Marine-Earth Science and Technology for microscopic observations using their HTM system during the initial stages of this study, and Dr. Haruyo Yamaguchi for helping us with electron microscopy techniques. We are also grateful to Mr. Akitomo Kawasaki for some TEM work.

Supplementary material

792_2012_481_MOESM1_ESM.doc (240 kb)
Supplementary material 1 (DOC 240 kb)

Supplementary material 2: Movie S2 (MPG 1647 kb)

Supplementary material 3: Movie S1 (MPG 4586 kb)

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Copyright information

© Springer 2012

Authors and Affiliations

  1. 1.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan

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