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Extremophiles

, Volume 16, Issue 2, pp 317–331 | Cite as

Effects of trace element concentrations on culturing thermophiles

  • D. R. Meyer-DombardEmail author
  • E. L. Shock
  • J. P. Amend
Original Paper

Abstract

The majority of microorganisms in natural environments resist laboratory cultivation. Sometimes referred to as ‘unculturable’, many phylogenetic groups are known only by fragments of recovered DNA. As a result, the ecological significance of whole branches of the ‘tree of life’ remains a mystery; this is particularly true when regarding genetic material retrieved from extreme environments. Geochemically relevant media have been used to improve the success of culturing Archaea and Bacteria, but these efforts have focused primarily on optimizing pH, alkalinity, major ions, carbon sources, and electron acceptor–donor pairs. Here, we cultured thermophilic microorganisms from ‘Sylvan Spring’ (Yellowstone National Park, USA) on media employing different trace element solutions, including one that mimicked the source fluid of the inocula. The growth medium that best simulated trace elements found in ‘Sylvan Spring’ produced a more diverse and faster growing mixed culture than media containing highly elevated trace element concentrations. The elevated trace element medium produced fewer phylotypes and inhibited growth. Trace element concentrations appear to influence growth conditions in extreme environments. Incorporating geochemical data into cultivation attempts may improve culturing success.

Keywords

Culturing Thermophiles Trace element composition Hydrothermal systems Microbial ecology 

Notes

Acknowledgments

This work was funded largely by a NASA Graduate Student Research Program (GSRP) fellowship (NGT5-50348) to D.R.M.D., also NSF-LExEN (OCE-9817730), and NASA Astrobiology Institute (Carnegie Institution) to E.L.S., and a NSF-CAREER grant (0447231) to J.P.A.

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Copyright information

© Springer 2012

Authors and Affiliations

  • D. R. Meyer-Dombard
    • 1
    Email author
  • E. L. Shock
    • 2
  • J. P. Amend
    • 3
  1. 1.Department of Earth and Environmental SciencesUniversity of Illinois at ChicagoChicagoUSA
  2. 2.School of Earth and Space Exploration, and Department of Chemistry and BiochemistryArizona State UniversityTempeUSA
  3. 3.Departments of Earth Sciences and Biological SciencesUniversity of Southern CaliforniaLos AngelesUSA

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