, 15:611 | Cite as

Bioprocessing of agricultural residues to ethanol utilizing a cellulolytic extremophile

  • Vasudeo P. Zambare
  • Aditya Bhalla
  • Kasiviswanath Muthukumarappan
  • Rajesh K. Sani
  • Lew P. ChristopherEmail author
Original Paper


A recently discovered thermophilic isolate, Geobacillus sp. R7, was shown to produce a thermostable cellulase with a high hydrolytic potential when grown on extrusion-pretreated agricultural residues such corn stover and prairie cord grass. At 70°C and 15–20% solids, the thermostable cellulase was able to partially liquefy solid biomass only after 36 h of hydrolysis time. The hydrolytic capabilities of Geobacillus sp. R7 cellulase were comparable to those of a commercial cellulase. Fermentation of the enzymatic hydrolyzates with Saccharomyces cerevisiae ATCC 24860 produced ethanol yields of 0.45–0.50 g ethanol/g glucose with more than 99% glucose utilization. It was further demonstrated that Geobacillus sp. R7 can ferment the lignocellulosic substrates to ethanol in a single step that could facilitate the development of a consolidated bioprocessing as an alternative approach for bioethanol production with outstanding potential for cost reductions.


Bioprocessing Agroresidues Bioethanol Cellulase Geobacillus Consolidated bioprocessing High solids Enzymatic hydrolysis 



Financial support by the Center for Bioprocessing R&D (CBRD) at the South Dakota School of Mines and Technology (SDSM&T), the South Dakota Board of Reagents (SD BOR) and the Governor’s Office for Economic Development (GOED) of South Dakota, USA, is gratefully acknowledged.


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Copyright information

© Springer 2011

Authors and Affiliations

  • Vasudeo P. Zambare
    • 1
  • Aditya Bhalla
    • 1
    • 2
  • Kasiviswanath Muthukumarappan
    • 3
  • Rajesh K. Sani
    • 1
    • 2
  • Lew P. Christopher
    • 1
    • 2
    Email author
  1. 1.Center for Bioprocessing Research and DevelopmentSouth Dakota School of Mines and TechnologyRapid CityUSA
  2. 2.Department of Chemical and Biological EngineeringSouth Dakota School of Mines and TechnologyRapid CityUSA
  3. 3.Department of Agricultural and Biosystems Engineering, Center for Bioprocessing Research and DevelopmentSouth Dakota State UniversityBrookingsUSA

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