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Extremophiles

, 15:517 | Cite as

The major lipid cores of the archaeon Ignisphaera aggregans: implications for the phylogeny and biosynthesis of glycerol monoalkyl glycerol tetraether isoprenoid lipids

  • Chris S. Knappy
  • Charlotte E. M. Nunn
  • Hugh W. Morgan
  • Brendan J. KeelyEmail author
Original Paper

Abstract

The lipid cores from Ignisphaera aggregans, a hyperthermophilic Crenarchaeon recently isolated from New Zealand hot springs, have been profiled by liquid chromatography–tandem mass spectrometry. The distribution revealed includes relatively high proportions of monoalkyl (also known as H-shaped) tetraether cores which have previously been implicated as kingdom-specific biomarkers for the Euryarchaeota. Such high expression of monoalkyl tetraether lipids is unusual in the archaeal domain and may indicate that formation of these components is an adaptive mechanism that allows I. aggregans to regulate membrane behaviour at high temperatures. The observed dialkyl tetraether and monoalkyl tetraether lipid distributions are similar but not fully concordant, showing differences in the average number of incorporated rings. The similarity supports a biosynthetic route to the ring-containing dialkyl and monoalkyl tetraether lipids via a dialkyl tetraether core containing zero rings, or a closely related structural relative, as an intermediate. Currently, however, the precise nature of the biosynthetic route to these lipids cannot be deduced.

Keywords

Ignisphaera Archaea Tetraether lipids H-shaped lipids Isoprenoids Tandem mass spectrometry 

Abbreviations

CID

Collision-induced dissociation

Cp

Cyclopentyl

Da

Daltons

DCM

Dichloromethane

GDD

Glycerol dialkyl diether

GDGT

Glycerol dialkyl glycerol tetraether

GMD

Glycerol monoalkyl diether

GMGT

Glycerol monoalkyl glycerol tetraether

GTGT

Glycerol trialkyl glycerol tetraether

LC–MS/MS

Liquid chromatography–tandem mass spectrometry

[M+H]+

Protonated molecule

APCI

Atmospheric pressure chemical ionisation

ESI

Electrospray ionisation

Notes

Acknowledgments

The authors acknowledge support for this study by a scholarship from the University of York. James Chong (University of York, UK; M. thermautotrophicus) and Michael Danson (University of Bath, UK; S. solfataricus and S. shibatae) are thanked for provision of the cellular material used to tune the mass spectrometer and verify the GDGT lipid assignments in I. aggregans, respectively. Two anonymous reviewers are also thanked for their constructive comments.

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Copyright information

© Springer 2011

Authors and Affiliations

  • Chris S. Knappy
    • 1
  • Charlotte E. M. Nunn
    • 2
  • Hugh W. Morgan
    • 3
  • Brendan J. Keely
    • 1
    Email author
  1. 1.Department of ChemistryUniversity of YorkYorkUK
  2. 2.Centre for Extremophile ResearchUniversity of BathBathUK
  3. 3.Thermophile Research UnitUniversity of WaikatoHamiltonNew Zealand

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