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Extremophiles

, Volume 15, Issue 2, pp 155–163 | Cite as

Autotrophic, sulfur-oxidizing actinobacteria in acidic environments

  • Paul R. Norris
  • Carol S. Davis-Belmar
  • Carly F. Brown
  • Leonides A. Calvo-Bado
Original Paper

Abstract

Some novel actinobacteria from geothermal environments were shown to grow autotrophically with sulfur as an energy source. These bacteria have not been formally named and are referred to here as “Acidithiomicrobium” species, as the first of the acidophilic actinobacteria observed to grow on sulfur. They are related to Acidimicrobium ferrooxidans with which they share a capacity for ferrous iron oxidation. Ribulose bisphosphate carboxylase/oxygenase (RuBisCO) is active in CO2 fixation by Acidimicrobium ferrooxidans, which appears to have acquired its RuBisCO-encoding genes from the proteobacterium Acidithiobacillus ferrooxidans or its ancestor. This lateral transfer of RuBisCO genes between a proteobacterium and an actinobacterium would add to those noted previously among proteobacteria, between proteobacteria and cyanobacteria and between proteobacteria and plastids. “Acidithiomicrobium” has RuBisCO-encoding genes which are most closely related to those of Acidimicrobium ferrooxidans and Acidithiobacillus ferrooxidans, and has additional RuBisCO genes of a different lineage. 16S rRNA gene sequences from “Acidithiomicrobium” species dominated clone banks of the genes extracted from mixed cultures of moderate thermophiles growing on copper sulfide and polymetallic sulfide ores in ore leaching columns.

Keywords

Acidophiles Actinobacteria Autotrophy Sulfur oxidation 

Notes

Acknowledgments

This work was funded by BHPBilliton PLC, The University of Warwick Overseas Research Student Fund and the European Commission under the Sixth Framework Programme for Research and Development (EU BioShale project, contract NMP2-CT-2004 505710).

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Copyright information

© Springer 2011

Authors and Affiliations

  • Paul R. Norris
    • 1
  • Carol S. Davis-Belmar
    • 1
    • 2
  • Carly F. Brown
    • 1
    • 3
  • Leonides A. Calvo-Bado
    • 1
  1. 1.School of Life SciencesUniversity of WarwickCoventryUK
  2. 2.BHPBilliton, c/o Biotechnology CenterUniversidad Catolica del NorteAntofagastaChile
  3. 3.Department of Plant SciencesUniversity of OxfordOxfordUK

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