Isolation of hydrocarbon-degrading extremely halophilic archaea from an uncontaminated hypersaline pond (Camargue, France)
- 775 Downloads
Little information exists about the ability of halophilic archaea present in hypersaline environments to degrade hydrocarbons. In order to identify the potential actors of hydrocarbon degradation in these environments, enrichment cultures were prepared using samples collected from a shallow crystallizer pond with no known contamination history in Camargue, France, with n-alkanes provided as source of carbon and energy. Five alkane-degrading halophilic archaeal strains were isolated: one (strain MSNC 2) was closely related to Haloarcula and three (strains MSNC 4, MSNC 14, and MSNC 16) to Haloferax. Biodegradation assays showed that depending on the strain, 32 to 95% (0.5 g/l) of heptadecane was degraded after 30 days of incubation at 40°C in 225 g/l NaCl artificial medium. One of the strains (MSNC 14) was also able to degrade phenanthrene. This work clearly shows for the first time the potential role of halophilic archaea belonging to the genera Haloarcula and Haloferax in the degradation of hydrocarbons in both pristine and hydrocarbon-contaminated hypersaline environments.
KeywordsHalophilic Archaea n-Alkanes Phenantrene Biodegradation Hypersaline ponds
The work was carried out as part of Yosmina Tapilatu’s PhD research and of the French National Program EC2CO “BIOHYDEX (BIOdégradation des HYDrocarbures dans les milieux EXtrêmes”). We thank the Centre National de la Recherche Scientifique (CNRS) and the Institut National des Sciences de l’Univers (INSU) for financial support. Y.T. was the recipient of a scholarship from the French Foreign Ministry.
- Bezalel L, Hadar Y, Fu PP, Freeman JP, Cerniglia (1996) Metabolism of phenanthrene by the white rot fungus Pleurotus ostreatus. Appl Environ Microbiol 62:2547–53Google Scholar
- Hadibarata T, Tachibana S (2009) Identification of phenanthrene metabolites produced by Polyporus sp. S133. In: Obayashi Y, Isobe T, Subramanian A, Suzuki S, Tanabe S (eds) Interdisciplinary studies on environmental chemistry-environmental research in Asia. Terrapub, Tokyo, pp 293–299Google Scholar
- Kulichevskaya IS, Milekhina EI, Borzenkov IA, Zvyagintseva IS, Belyaev SS (1991) Oxidation of petroleum hydrocarbons by extremely halophilic archeobacteria. Microbiology 60:596–601Google Scholar
- Lefebvre O (2005) Application des micro-organismes halophiles au traitement des effluents industriels hypersalins. Ph.D Thesis. Ecole Nationale Supérieure Agronomique de Montpellier, p. 12Google Scholar
- Malachowsky KJ, Phelps TJ, Teboli AB, Minnikin DE, White DC (1994) Aerobic mineralization of trichloroethylene, vinyl chloride, and aromatic compounds by Rhodococcus species. Appl Environ Microbiol 60:42–548Google Scholar
- Patzelt H (2005) Hydrocarbon degradation under hypersaline conditions––some facts, some experiments and many open questions. In: Gunde-Cimerman N, Oren A, Plemenitas A (eds) Adaptation to life at high salt concentrations in archaea bacteria and eukarya. Springer, Berlin, pp 105–122CrossRefGoogle Scholar
- Robinson JL, Pyzyna B, Atrasz RG, Henderson CA, Morrill KL, Burd AM, DeSoucy E, Fogleman RE III, Naylor JB, Steele SM, Elliott DR, Leyva KJ, Shand RF (2005) Growth kinetics of extremely halophilic Archaea (family Halobacteriaceae) as revealed by arrhenius plots. J Bacteriol 187:923–929CrossRefPubMedGoogle Scholar
- Seo J-S, Keum Y-S, Hu Y, Lee S-E, Li QX (2006) Phenanthrene degradation in Arthrobacter sp. P1-1: initial 1,2-, 3,4- and 9,10-dioxygenation, and meta- and ortho-cleavages of naphthalene-1, 2-diol after its formation from naphthalene-1,2-dicarboxylic acid and hydroxyl naphthoic acids. Chemosphere 65:2388–2394CrossRefPubMedGoogle Scholar
- Speight JG (1998) The chemistry and technology of petroleum. Marcel Dekker, New YorkGoogle Scholar
- Torreblanca M, Rodriguez-Valera F, Juez G, Ventosa A, Kamekura M, Kates M (1986) Classification of non-alkaliphilic halobacteria based on numerical taxonomy and polar lipid composition, and description of Haloarcula gen nov. and Haloferax gen. nov. System Appl Microbiol 8:89–99Google Scholar
- Zvyagintseva I, Belyaev S, Borzenkov I, Kostrikina N, Milekhina E, Ivanov M (1995) Halophilic archaebacteria from the Kalamkass oil field. Microbiology 64:67–71Google Scholar