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Extremophiles

, Volume 13, Issue 6, pp 867–874 | Cite as

Anoxybacillus thermarum sp. nov., a novel thermophilic bacterium isolated from thermal mud in Euganean hot springs, Abano Terme, Italy

  • Annarita PoliEmail author
  • Ida Romano
  • Paolo Cordella
  • Pierangelo Orlando
  • Barbara Nicolaus
  • Cristina Ceschi Berrini
Original Paper

Abstract

A novel aerobe thermophilic endospore-forming bacterium designated strain AF/04T was isolated from thermal mud located in Euganean hot springs, Abano Terme, Padova, Italy. Strain AF/04T was Gram-positive, motile, rod-shaped, occurring in pairs, or filamentous. The isolate grew between 55 and 67°C (optimum 65°C) and at pH 6.0–7.5 (optimum pH 7.2). The strain was aerobic and grew on maltose, trehalose, and sodium acetate as sole carbon sources. The G + C content of DNA was 53.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AF/04T falls within the genus Anoxybacillus. Levels of 16S rRNA gene sequence similarity between strain AF/04T and the type strains of recognized Anoxybacillus species ranged from 95 to 99%. Chemotaxonomic data (major isoprenoid quinone–menaquinone-7; major fatty acid iso-C15:0 and anteiso-C17:0) supported the affiliation of strain AF/04T to the genus Anoxybacillus. Based on phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence analysis and DNA–DNA hybridization data, it was proposed that strain AF/04T (=DSM 17141T = ATCC BAA 1156T) should be placed in the genus Anoxybacillus as the type strain of a novel species, Anoxybacillus thermarum sp. nov.

Keywords

Anoxybacillus Taxonomy Thermophilic Lipid DNA–DNA hybridisation 16S rRNA analysis Euganean hot spring 

Notes

Acknowledgments

The authors thank Valeria Calandrelli e Raffaele Turco for technical assistance and Dominique Melk for NMR-ICB service. We are grateful to Prof. J.P. Euzeby, who kindly helped in the correct epithet of the new species.

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Copyright information

© Springer 2009

Authors and Affiliations

  • Annarita Poli
    • 1
    Email author
  • Ida Romano
    • 1
  • Paolo Cordella
    • 2
  • Pierangelo Orlando
    • 3
  • Barbara Nicolaus
    • 1
  • Cristina Ceschi Berrini
    • 2
  1. 1.Istituto di Chimica Biomolecolare (ICB), CNRPozzuoli (Naples)Italy
  2. 2.Dipartimento di BiologiaUniversità di PadovaPadovaItaly
  3. 3.Istituto di Biochimica delle Proteine (IBP), CNRNaplesItaly

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