Effect of temperature on growth parameters of psychrophilic bacteria and yeasts
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Three bacterial (Pedobacter heparinus, Pedobacter piscium, Pedobacter cryoconitis) and three yeast strains (Saccharomyces cerevisiae, Leucosporidiella creatinivora, Rhodotorula glacialis) of different thermal classes (mesophiles and psychrophiles) were tested for the effect of temperature on a range of growth parameters, including optical density, viable cell numbers, and cell dry mass, in order to determine the temperature conditions under which maximum biomass formation is obtained. Maximum values of growth parameters obtained at the stationary growth phase of the strains were used for statistical calculation. Temperature had a significant (P ≤ 0.05) effect on all growth parameters for each strain; correlations between the growth parameters were significant (P ≤ 0.05–0.01). The maximum growth temperature or the temperature at which microbial growth was fastest was in no case the temperature at which the investigated strains produced the highest amount of biomass. All tested psychrophilic bacteria and yeast strains produced highest amounts of cells (as calculated per mg cell dry mass or per OD600 unit) at 1°C, while cell numbers of mesophiles were highest at 20°C. Thus, cultivation temperatures close to the maximum growth temperature are not appropriate for studying psychrophiles.
KeywordsPsychrophilic Growth temperature Bacteria Yeasts
The author thanks Karin Weber for technical assistance.
- Feller G, Narinx E, Arpigny JL, Zekhnini Z, Swings J, Gerday C (1994) Temperature dependence of growth, enzyme secretion and activity of psychrophilic Antarctic bacteria. Appl Microbiol Biotechnol 41:477–479Google Scholar
- Gerday C (2008) On life in the cold: approximations and mistakes. In: Program and abstracts, 3rd international conference on polar and alpine microbiology, Banff, Canada, May 11–15, 2008, p 24Google Scholar
- Glansdorff N, Xu Y (2002) Microbial life at low temperatures: mechanisms of adaptation and extreme biotopes. Implications for exobiology and the origin of life. Recent Res Dev Microbiol 6:1–21Google Scholar
- Gounot AM, Russell NJ (1999) Physiology of cold-adapted microorganisms. In: Margesin R, Schinner F (eds) Cold-adapted organisms. Springer, Berlin, pp 33–55Google Scholar
- Koch AL (1994) Growth measurement. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. ASM Press, Washington DC, pp 248–292Google Scholar
- Margesin R, Fonteyne PA, Schinner F, Sampaio JP (2007) Rhodotorula psychrophila sp. nov., Rhodotorula psychrophenolica sp. nov. and Rhodotorula glacialis sp. nov., novel psychrophilic basidiomycetous yeast species from alpine environments. Int J Syst Evol Microbiol 57:2179–2184PubMedCrossRefGoogle Scholar
- Margesin R, Schinner F, Marx JC, Gerday C (eds) (2008) Psychrophiles from biodiversity to biotechnology. Springer, Berlin, p 462Google Scholar
- Steyn PL, Seger P, Vancanneyt M, Kersters K, Joubert JJ (1998) Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. Proposal of the family Sphingobacteriaceae fam. nov. Int J Syst Microbiol 48:165–177CrossRefGoogle Scholar