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Extremophiles

, Volume 13, Issue 2, pp 247–255 | Cite as

Desulfonauticus autotrophicus sp. nov., a novel thermophilic sulfate-reducing bacterium isolated from oil-production water and emended description of the genus Desulfonauticus

  • Shanmugam Mayilraj
  • Anna H. Kaksonen
  • Ralf Cord-Ruwisch
  • Peter Schumann
  • Cathrin Spröer
  • Brian J. Tindall
  • Stefan SpringEmail author
Original Paper

Abstract

A novel moderately thermophilic and halophilic sulfate-reducing bacterium, strain TeStT, was isolated from production water of an oil field in Northern Germany near Hamburg. The cells were Gram-negative, straight to slightly curved rods and motile by a single polar flagellum. Only hydrogen and formate served as electron donors, whereas a wide variety of organic substrates and CO2 could be used as carbon sources. Sulfate, sulfite, thiosulfate and sulfur were used as electron acceptors, but not nitrate or ferric iron. The novel isolate was negative for oxidase, catalase and desulfoviridin enzyme activity. Cytochromes were present and predominantly of the c-type. Whole-cells fatty acid patterns were dominated by the branched-chain fatty acids anteiso-C15:0, iso-C15:0, iso-C17:0 and anteiso-C17:0. As major respiratory lipoquinones partially saturated derivates of menaquinone 6 [MK-6(H2) and probably MK-6(H4)] were identified. The G + C content of the genomic DNA was 41.3 mol% (HPLC method). An analysis of the 16S rRNA gene sequence indicated that strain TeStT belongs to the family Desulfohalobiaceae within the class Deltaproteobacteria. The most closely related species with a sequence similarity of 95.0% was Desulfonauticus submarinus suggesting an affiliation of TeStT to the genus Desulfonauticus. The novel isolate could be clearly distinguished from Desulfonauticus submarinus by its ability to grow chemolithoautotrophically and hence should be assigned to a novel species for which the name Desulfonauticus autotrophicus sp. nov. is proposed. The type strain is TeStT (=DSM 4206T = JCM 13028T).

Keywords

Desulfohalobiaceae Sulfate reduction Chemolithoautotroph Hydrogenotroph Novel menaquinone Oil production 

Notes

Acknowledgments

Ina Kramer and Jennifer Gregor are acknowledged for determining the 16S rRNA gene sequence of strain TeStT. The cellular fatty acid profiles were determined by Marlen Jando and Gabriele Pötter. Shanmugam Mayilraj acknowledges a bilateral exchange program awarded by INSA (India) and DFG (Germany). The Academy of Finland is acknowledged for the financial support for Anna H. Kaksonen for the project “Deep biosphere microbiology” (Decision no. 122394).

Supplementary material

792_2008_212_MOESM1_ESM.pdf (66 kb)
Supplementary table and figure (PDF 65 kb)

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Copyright information

© Springer 2008

Authors and Affiliations

  • Shanmugam Mayilraj
    • 1
  • Anna H. Kaksonen
    • 2
  • Ralf Cord-Ruwisch
    • 3
  • Peter Schumann
    • 4
  • Cathrin Spröer
    • 4
  • Brian J. Tindall
    • 4
  • Stefan Spring
    • 4
    Email author
  1. 1.Microbial Type Culture Collection and Gene Bank (MTCC)Institute of Microbial TechnologyChandigarhIndia
  2. 2.Department of Chemistry and BioengineeringTampere University of TechnologyTampereFinland
  3. 3.School of Biological Sciences and BiotechnologyMurdoch UniversityPerthAustralia
  4. 4.DSMZ—German Collection of Microorganisms and Cell CulturesBraunschweigGermany

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