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Extremophiles

, 13:371 | Cite as

A PPM-family protein phosphatase from the thermoacidophile Thermoplasma volcanium hydrolyzes protein-bound phosphotyrosine

  • Hanan Dahche
  • AbdulShakur Abdullah
  • M. Ben Potters
  • Peter J. Kennelly
Note

Abstract

The genomes of virtually all free-living archaeons encode one or more deduced protein-serine/threonine/tyrosine kinases belonging to the so-called eukaryotic protein kinase superfamily. However, the distribution of their cognate protein-serine/threonine/phosphatases displays a mosaic pattern. Thermoplasma volcanium is unique among the Archaea inasmuch as it is the sole archaeon whose complement of deduced phosphoprotein phosphatases includes a member of the PPM-family of protein-serine/threonine phosphatases—a family that originated in the Eucarya. A recombinant version of this protein, TvnPPM, exhibited protein-tyrosine phosphatase in addition to its predicted protein-serine/threonine phosphatase activity in vitro. TvnPPM is the fourth member of the PPM-family shown to exhibit such dual-specific capability, suggesting that the ancestral versions of this enzyme exhibited broad substrate specificity. Unlike most other archeaons, the genome of T. volcanium lacks open reading frames encoding stereotypical protein-tyrosine phosphatases. Hence, the dual-specificity of TvnPPM may account for its seemingly aberrant presence in an archaeon.

Keywords

Protein phosphorylation Signal transduction Dual-specific protein phosphatase Archaea Protein-tyrosine phosphatase Protein-serine/threonine phosphatase Zinc 

Notes

Acknowledgments

This work was supported by grant MCB-0315122 from the National Science Foundation.

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Copyright information

© Springer 2008

Authors and Affiliations

  • Hanan Dahche
    • 1
  • AbdulShakur Abdullah
    • 1
  • M. Ben Potters
    • 1
  • Peter J. Kennelly
    • 1
  1. 1.Department of BiochemistryVirginia Polytechnic Institute and State UniversityBlacksburgUSA

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