Fatty acids, unusual glycophospholipids and DNA analyses of thermophilic bacteria isolated from hot springs
The composition of fatty acids in 12 strains of the genera Thermus, Meiothermus, Geobacillus and Alicyclobacillus was analyzed by gas chromatography–mass spectrometry. Major FAs found in the profiles included i-15:0, i-17:0, ai-15:0, i-16:0, 16:0, ai-17:0, together with some minor components. Branched FAs were predominant, forming more than 80% of all FAs measured. Fast atom bombardment-mass spectrometry was used for analysis of unusual glycophospholipids, i.e., acylglycosylcardiolipins from genera Geobacillus and Alicyclobacillus and 1-(hydroxy(2-(O-acylglycosyl-oxy)hexadecyloxy)phosphoryloxy) hexadecan-2-yl esters of C15–C17 acids from genera Thermus and Meiothermus. Cloning and preliminary sequence analysis of 16S rDNA showed that these isolates belong to the genera Thermus, Meiothermus, Geobacillus and Alicyclobacillus.
KeywordsThermus Meiothermus Geobacillus Alicyclobacillus Fatty acids Glycophospholipids FAB-MS 16S rDNA analysis Phylogenetic tree Hot springs
The research was supported by Institutional Research Concept AV 0Z 502 0910 and by the project No. MSM6046137305 of the Ministry of Education, Youth and Sport of the Czech Republic.
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