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Extremophiles

, Volume 12, Issue 3, pp 391–404 | Cite as

Influence of salts and pH on growth and activity of a novel facultatively alkaliphilic, extremely salt-tolerant, obligately chemolithoautotrophic sufur-oxidizing Gammaproteobacterium Thioalkalibacter halophilus gen. nov., sp. nov. from South-Western Siberian soda lakes

  • Horia L. BanciuEmail author
  • Dimitry Y. Sorokin
  • Tatyana P. Tourova
  • Erwin A. Galinski
  • Maria S. Muntyan
  • J. Gijs Kuenen
  • Gerard Muyzer
Original Paper

Abstract

A chemolithoautotrophic sulfur-oxidizing bacterium (SOB) strain ALCO 1 capable of growing at both near-neutral and extremely alkaline pH was isolated from hypersaline soda lakes in S-W Siberia (Altai, Russia). Strain ALCO 1 represents a novel separate branch within the halothiobacilli in the Gammaproteobacteria, which, so far, contained only neutro-halophilic SOB. On the basis of its unique phenotypic properties and distant phylogeny, strain ALCO 1 is proposed as a new genus and species Thioalkalibacter halophilus gen. nov. sp. nov. ALCO 1 was able to grow within a broad range of salinity (0.5–3.5 M of total sodium) with an optimum at around 1 M Na+, and pH (7.2–10.2, pHopt at around 8.5). Na+ was required for sulfur-dependent respiration in ALCO 1. The neutral (NaCl)-grown chemostat culture had a much lower maximum growth rate (μmax), respiratory activity and total cytochrome c content than its alkaline-grown counterpart. The specific concentration of osmolytes (ectoine and glycine-betaine) produced at neutral pH and 3 M NaCl was roughly two times higher than at pH 10 in soda. Altogether, strain ALCO 1 represents an interesting chemolithoautotrophic model organism for comparative investigations of bacterial adaptations to high salinity and pH.

Keywords

Haloalkaliphilic Halothiobacillus Soda lakes Sulfur-oxidizing bacteria Thioalkalibacter halophilus 

Notes

Acknowledgments

This work was supported by the NWO-RFBR (grant 47.011.2004.010), RFBR (grants 07-04-00153 and 05-04-49504), by the Program of the Russian Academy of Sciences “Molecular and Cell Biology” and by the Program for Excellence in Research of Romanian Ministry for Research and Education (CEEX-ET, Nr. 5913/18.09.2006).

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Copyright information

© Springer 2008

Authors and Affiliations

  • Horia L. Banciu
    • 1
    Email author
  • Dimitry Y. Sorokin
    • 2
    • 3
  • Tatyana P. Tourova
    • 2
  • Erwin A. Galinski
    • 4
  • Maria S. Muntyan
    • 5
  • J. Gijs Kuenen
    • 3
  • Gerard Muyzer
    • 3
  1. 1.Faculty of Biology and GeologyBabes-Bolyai UniversityCluj-NapocaRomania
  2. 2.S. N. Winogradsky Institute of MicrobiologyRussian Academy of SciencesMoscowRussia
  3. 3.Department of BiotechnologyDelft University of TechnologyDelftThe Netherlands
  4. 4.Institute of Microbiology and BiotechnologyRheinische Friedrich-Wilhelms UniversityBonnGermany
  5. 5.A. N. Belozersky Institute of Physico-Chemical BiologyMoscow State UniversityMoscowRussia

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