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Extremophiles

, Volume 11, Issue 6, pp 789–796 | Cite as

Halomonas sinaiensis sp. nov., a novel halophilic bacterium isolated from a salt lake inside Ras Muhammad Park, Egypt

  • Ida Romano
  • Licia Lama
  • Pierangelo Orlando
  • Barbara Nicolaus
  • Assunta Giordano
  • Agata GambacortaEmail author
Original Paper

Abstract

An alkalitolerant and halotolerant bacterium, designated strain Sharm was isolated from a salt lake inside Ras Muhammad. The morphological, physiological and genetic characteristics were compared with those of related species of the genus Halomonas. The isolate grew optimally at pH 7.0, 5–15% NaCl at 35°C. The cells were Gram-negative rods, facultative anaerobes. They accumulated glycine-betaine, as a major osmolyte, and ectoine and glutamate as minor components. The strain SharmT biosynthetised α-glucosidase. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and a novel phosphoglycolipid as major components. Ubiquinone with nine repetitive unities (Q9) was the only quinone found and, nC16:0 and C19:0 with cyclopropane were the main cellular fatty acids, accounting for 87.3% of total fatty acids. The G + C content of the genomic DNA was 64.7 mol %. The 16S rRNA sequence analysis indicated that strain Sharm was a member of the genus Halomonas. The closest relatives of the strain Sharm were Halomonas elongata and Halomonas eurihalina. However, DNA–DNA hybridisation results clearly indicated that strain Sham was a distinct species of Halomonas. On the basis of the evidence, we propose to assign strain Sharm as a new species of the genus Halomonas, H. sinaiensis sp. nov, with strain SharmT as the type strain (DSM 18067T; ATCC BAA-1308T).

Keywords

Egypt Extremophiles Halomonas Halophiles Lipids 

Notes

Acknowledgment

The paper was supported by the framework PNRA. The authors thank Valeria Calandrelli, Eduardo Pagnotta for technical assistance, Vincenzo Mirra, Salvatore Zambardino and Dominique Merk for NMR-ICB service, Ottavio De Luca for GC-MS analyses and Emilio P. Castelluccio for computer system maintenance, and Dr. F.M. Vella for some experiments.

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Copyright information

© Springer 2007

Authors and Affiliations

  • Ida Romano
    • 1
  • Licia Lama
    • 1
  • Pierangelo Orlando
    • 2
  • Barbara Nicolaus
    • 1
  • Assunta Giordano
    • 1
  • Agata Gambacorta
    • 1
    Email author
  1. 1.Istituto di Chimica Biomolecolare, Comprensorio ex OlivettiNapoliItaly
  2. 2.Istituto di Biochimica delle Proteine, CNRNapoliItaly

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