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Extremophiles

, Volume 11, Issue 5, pp 719–732 | Cite as

Molecular characterization of cold adaptation based on ortholog protein sequences from Vibrionaceae species

  • Steinar ThorvaldsenEmail author
  • Erik Hjerde
  • Chris Fenton
  • Nils P. Willassen
Original Paper

Abstract

A set of 298 protein families from psychrophilic Vibrio salmonicida was compiled to identify genotypic characteristics that discern it from orthologous sequences from the mesophilic Vibrio/Photobacterium branch of the gamma-Proteobacteria (Vibrionaceae family). In our comparative exploration we employed alignment based bioinformatical and statistical methods. Interesting information was found in the substitution matrices, and the pattern of asymmetries in the amino acid substitution process. Together with the compositional difference, they identified the amino acids Ile, Asn, Ala and Gln as those having the most psycrophilic involvement. Ile and Asn are enhanced whereas Gln and Ala are suppressed. The inflexible Pro residue is also suppressed in loop regions, as expected in a flexible structure. The dataset were also classified and analysed according to the predicted subcellular location, and we made an additional study of 183 intracellular and 65 membrane proteins. Our results revealed that the psychrophilic proteins have similar hydrophobic and charge contributions in the core of the protein as mesophilic proteins, while the solvent-exposed surface area is significantly more hydrophobic. In addition, the psychrophilic intracellular (but not the membrane) proteins are significantly more negatively charged at the surface. Our analysis supports the hypothesis of preference for more flexible amino acids at the molecular surface. Life in cold climate seems to be obtained through many minor structural modifications rather than certain amino acids substitutions.

Keywords

Psychrophiles Vibrio Comparative genomics Substitution matrix Physicochemical properties 

Notes

Acknowledgments

The present study was supported by the National Program for Research in Functional Genomics in Norway (FUGE). The Matlab toolbox DeltaProt (http://www.math.uit.no/bi/deltaprot/) was used for the data analyses.

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Copyright information

© Springer 2007

Authors and Affiliations

  • Steinar Thorvaldsen
    • 1
    Email author
  • Erik Hjerde
    • 2
  • Chris Fenton
    • 2
  • Nils P. Willassen
    • 2
    • 3
  1. 1.Department of Mathematics and Statistics, Faculty of ScienceUniversity of TromsøTromsøNorway
  2. 2.Department of Molecular Biotechnology, Faculty of MedicineUniversity of TromsøTromsøNorway
  3. 3.The Norwegian Structural Biology CentreUniversity of TromsøTromsøNorway

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