Extremophiles

, Volume 11, Issue 2, pp 343–354

Proteomic analysis of Psychrobacter cryohalolentis K5 during growth at subzero temperatures

  • Corien Bakermans
  • Sandra L. Tollaksen
  • Carol S. Giometti
  • Curtis Wilkerson
  • James M. Tiedje
  • Michael F. Thomashow
Original Paper

Abstract

It is crucial to examine the physiological processes of psychrophiles at temperatures below 4°C, particularly to facilitate extrapolation of laboratory results to in situ activity. Using two dimensional electrophoresis, we examined patterns of protein abundance during growth at 16, 4, and −4°C of the eurypsychrophile Psychrobacter cryohalolentis K5 and report the first identification of cold inducible proteins (CIPs) present during growth at subzero temperatures. Growth temperature substantially reprogrammed the proteome; the relative abundance of 303 of the 618 protein spots detected (∼31% of the proteins at each growth temperature) varied significantly with temperature. Five CIPs were detected specifically at −4°C; their identities (AtpF, EF-Ts, TolC, Pcryo_1988, and FecA) suggested specific stress on energy production, protein synthesis, and transport during growth at subzero temperatures. The need for continual relief of low-temperature stress on these cellular processes was confirmed via identification of 22 additional CIPs whose abundance increased during growth at −4°C (relative to higher temperatures). Our data suggested that iron may be limiting during growth at subzero temperatures and that a cold-adapted allele was employed at −4°C for transport of iron. In summary, these data suggest that low-temperature stresses continue to intensify as growth temperatures decrease to −4°C.

Keywords

Psychrophiles Proteome Low-temperature Psychrobacter Cold acclimation 

Abbreviations

CIP

Cold inducible protein

CAP

Cold acclimation protein

CSP

Cold shock protein

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Copyright information

© Springer 2006

Authors and Affiliations

  • Corien Bakermans
    • 1
  • Sandra L. Tollaksen
    • 2
  • Carol S. Giometti
    • 2
  • Curtis Wilkerson
    • 3
  • James M. Tiedje
    • 1
  • Michael F. Thomashow
    • 1
  1. 1.Center for Microbial EcologyMichigan State UniversityEast LansingUSA
  2. 2.Biosciences DivisionArgonne National LaboratoryArgonneUSA
  3. 3.Research Technology Support FacilityMichigan State UniversityEast LansingUSA

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