Extremophiles

, Volume 11, Issue 2, pp 217–224 | Cite as

A novel highly boron tolerant bacterium, Bacillus boroniphilus sp. nov., isolated from soil, that requires boron for its growth

Original Paper

Abstract

Three strains of gram-positive, motile, rod-shaped and boron (B)-tolerant bacterium were isolated from naturally B containing soil of Hisarcik area in the Kutahya Province, Turkey. The strains, designated as T-14A, T-15ZT and T-17s, produced spherical or ellipsoidal endospores in a terminal bulging sporangium. The strains required B for the growth and can tolerate more than 450 mM B. These also tolerated up to 7.0% (w/v) NaCl in the presence of 50 mM B in agar medium but grew optimally without NaCl. The temperature range for growth was 16–37°C (optimal of 30°C), whereas the pH range was 6.5–9.0 (optimal of 7.5–8.5). The DNA G + C content was 41.1–42.2 mol% and the predominant cellular fatty acid was iso-C15:0. The major respiratory quinone system was detected as MK-7 and the diamino acid of the peptidoglycan was meso-diaminopimelic acid. Based on phenotypic and chemotaxonomic characteristics, phylogenetic analysis of 16S rRNA gene sequences data and DNA–DNA re-association values, we concluded that the three strains belong to a novel species of the genus Bacillus, the type strain of which is T-15ZT and for which we proposed the name, B. boroniphilus sp. nov. (DSM 17376T = IAM 15287T = ATCC BAA-1204T).

Keywords

Bacillus boroniphilus Boron tolerance Boron requirement Boron contaminated soil Hisarcik area Taxonomy 

Abbreviations

B

Boron

Nts

Nucleotides

TSB

Tryptic soya broth

TSA

Tryptic soya agar

PBS

Phosphate buffered saline solution

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Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Biotechnology Research CentreUniversity of TokyoTokyoJapan
  2. 2.National Agricultural Research CentreIslamabadPakistan
  3. 3.Institute of Molecular and Cellular BiosciencesUniversity of TokyoTokyoJapan
  4. 4.SORST, JSTTokyoJapan

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