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Extremophiles

, Volume 9, Issue 2, pp 111–116 | Cite as

Natronolimnobius baerhuensis gen. nov., sp. nov. and Natronolimnobius innermongolicus sp. nov., novel haloalkaliphilic archaea isolated from soda lakes in Inner Mongolia, China

  • Takashi ItohEmail author
  • Takashi Yamaguchi
  • Peijin Zhou
  • Tomonori Takashina
Original Paper

Abstract

Three novel isolates of haloalkaliphilic archaea, strains IHC-005T, IHC-010, and N-1311T, from soda lakes in Inner Mongolia, China, were characterized to elucidate their taxonomic positions. The three strains were aerobic, Gram-negative chemoorganotrophs growing optimally at 37–45°C, pH 9.0–9.5, and 15–20% NaCl. Cells of strains IHC-005T/IHC-010 were motile rods, while those of strain N-1311T were non-motile pleomorphic flats or cocci. The three strains contained diphytanyl and phytanyl-sesterterpanyl diether derivatives of phosphatidylglycerol and phosphatidylglycerophosphate methyl ester. No glycolipids were detected. On phylogenetic analysis of 16S rRNA gene sequences, they formed an independent cluster in the Natro group of the family Halobacteriaceae. Comparison of their morphological, physiological, and biochemical properties, DNA G+C content and 16S rRNA gene sequences, and DNA-DNA hybridization study support the view that strains IHC-005T/IHC-010 and strain N-1311T represent separate species. Therefore, we propose Natronolimnobius baerhuensis gen. nov., sp. nov. for strains IHC-005T (=CGMCC 1.3597T =JCM 12253T)/IHC-010 (=CGMCC 1.3598=JCM 12254) and Natronolimnobius innermongolicus sp. nov. for N-1311T (=CGMCC 1.2124T =JCM 12255T).

Keywords

Archaea Haloalkaliphile Halobacteriaceae Natronolimnobiusbaerhuensis Natronolimnobius innermongolicus Taxonomy 

Notes

Acknowledgements

We thank to Prof. You-xin Song for arranging the sampling trip to Hailar, China, and Dr. Huawei Xin for providing the preliminary taxonomic data of strain N-1311T.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Takashi Itoh
    • 1
    Email author
  • Takashi Yamaguchi
    • 1
    • 2
  • Peijin Zhou
    • 3
  • Tomonori Takashina
    • 2
  1. 1.Japan Collection of MicroorganismsRIKEN BioResource CenterWako, SaitamaJapan
  2. 2.Faculty of Life SciencesToyo UniversityItakura, GunmaJapan
  3. 3.The Institute of MicrobiologyChinese Academy of Sciences (CAS)BeijingP.R. China

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