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Extremophiles

, Volume 8, Issue 1, pp 63–71 | Cite as

Diversity of Kenyan soda lake alkaliphiles assessed by molecular methods

  • Helen C. Rees
  • William D. Grant
  • Brian E. Jones
  • Shaun Heaphy
Original Paper

Abstract

DNA was extracted from water and sediment samples taken from soda lakes of the Kenyan-Tanzanian Rift Valley. DNA was also extracted from microbial enrichment cultures of sediment samples. 16S rRNA genes were amplified by the polymerase chain reaction and microbial diversity was studied using denaturing gradient gel electrophoresis (DGGE) of 16S rDNA amplicons. Cloning and sequencing of single DGGE bands showed that they usually contained mixed amplicons. Several of the amplicon sequences had high identities, up to 99%, with 16S rRNA genes of organisms previously isolated from soda lakes, while others were only distantly related, with identities as low as 82%. Phylogenetic analysis of the sequenced amplicons indicated that sequences were related to the haloarchaeal, Bacillus/Clostridium, Rhodobacterium/Thioalcalovibrio/ Methylobacter, and Cytophaga/Flavobacterium/Bacteroides (CFB) groups and the enterobacteria/Aeromonas/Vibrio part of the γ3 subdivision of the Proteobacteria.

Keywords

16S rDNA Alkaliphile Denaturing gradient gel electrophoresis Phylogenetics Soda lake 

Notes

Acknowledgements

This study was supported by a committee studentship from the Biotechnology and Biological Sciences Research Council to H.R.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Helen C. Rees
    • 1
    • 3
  • William D. Grant
    • 1
  • Brian E. Jones
    • 2
  • Shaun Heaphy
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of LeicesterLeicesterUK
  2. 2.Genencor International BVLeidenThe Netherlands
  3. 3.Department of Civil and Structural EngineeringUniversity of SheffieldSheffieldUK

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