Extremophiles

, Volume 7, Issue 5, pp 385–390

Mode of action and antifungal properties of two cold-adapted chitinases

  • Konstantinos Mavromatis
  • Matteo Lorito
  • Sheridan L. Woo
  • Vassilis Bouriotis
Original Paper

DOI: 10.1007/s00792-003-0338-3

Cite this article as:
Mavromatis, K., Lorito, M., Woo, S.L. et al. Extremophiles (2003) 7: 385. doi:10.1007/s00792-003-0338-3

Abstract

The mode of action of two chitinases from the Antarctic Arthrobacter sp. strain TAD20 on N-acetyl-chitooligomers and chitin polymers has been elucidated. Identification of the length of chitin oligomers following enzymatic hydrolysis was verified by using HPLC-based analysis. It was observed that the length of the oligomer is important for enzyme action. The enzymes cannot effectively hydrolyze chitin oligomers with a degree of polymerization lower than four. ArChiA is an endochitinase which hydrolyzes chitin substrates randomly, whereas ArChiB is an exochitinase which degrades chitin chains and N-acetyl-chitooligomers from the nonreducing end, releasing N-N′-diacetyl-chitobiose. ArChiB (100 μg/ml) inhibited spore germination and hyphal elongation of the phytopathogenic fungus Botrytis cinerea by 15% and 30%, respectively. A more pronounced effect was observed with ArChiA (100 μg/ml) resulting in 70% inhibition of spore germination and 60% inhibition of germ tube elongation. A slight additive effect was observed, when the two enzymes were used in combination, only on the inhibition of germ tube elongation.

Keywords

Anomer separation Botrytis cinerea Chitinase Chitooligomers Endochitinase Exochitinase 

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Konstantinos Mavromatis
    • 1
    • 2
  • Matteo Lorito
    • 3
  • Sheridan L. Woo
    • 3
  • Vassilis Bouriotis
    • 1
    • 2
  1. 1.Department of Biology, Division of Applied Biology and BiotechnologyUniversity of CreteHeraklionGreece
  2. 2.Enzyme Technology DivisionInstitute of Molecular Biology and BiotechnologyHeraklionGreece
  3. 3.Dipartmento di Arboricoltura, Botanica e Patologia Vegetale, Sezione Patologia VegetaleUniversita degli Studi di Napoli Federico IIPorticiItaly

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