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Extremophiles

, Volume 7, Issue 5, pp 377–384 | Cite as

Isolation and characterization of marine psychrophilic phage-host systems from Arctic sea ice

  • Michael Borriss
  • Elisabeth Helmke
  • Renate Hanschke
  • Thomas SchwederEmail author
Original Paper

Abstract

Phage-host systems from extreme cold environments have rarely been surveyed. This study is concerned with the isolation and characterization of three different phage-host systems from Arctic sea ice and melt pond samples collected north-west of Svalbard (Arctic). On the basis of 16S rDNA sequences, the three bacterial phage hosts exhibited the greatest similarity to the species Shewanella frigidimarina (96.0%), Flavobacterium hibernum (94.0%), and Colwellia psychrerythraea (98.4%), respectively. The host bacteria are psychrophilic with good growth at 0°C, resulting in a rapid formation of visible colonies at this temperature. The phages showed an even more pronounced adaptation to cold temperatures than the bacteria, with growth maxima below 14°C and good plaque formation at 0°C. Transmission electron microscopy (TEM) examinations revealed that the bacteriophages belonged to the tailed, double-stranded DNA phage families Siphoviridae and Myoviridae. All three phages were host-specific.

Keywords

Marine bacteria Phages Psychrophilic Sea ice 

Notes

Acknowledgment

This work was supported by the Bundesministerium für Bildung und Forschung (03F0278B).

References

  1. Ausubel FM, et al (2001) Current protocols in molecular biology. Wiley: New YorkGoogle Scholar
  2. Bergh O, Boersheim KY, Bratbak G, Heldal M (1989) High abundance of viruses found in aquatic environments. Nature 6233:467–468CrossRefGoogle Scholar
  3. Bird DF, Maranger R, Karl DM (1993) Palmer LTER: aquatic virus abundances near the Antarctic Peninsula. Antarct J US 28:234–235Google Scholar
  4. Bowman JP, McCammon SA, Brown MV, Nichols DS, McMeekin TA (1997) Diversity and association of psychrophilic bacteria in Antarctic sea ice. Appl Environ Microbiol 63:3068–3078PubMedGoogle Scholar
  5. Bratbak G, Heldal M, Thingstad TF, Tuomi P (1996) Dynamics of virus abundance in coastal seawater. FEMS Microbiol Ecol 19:263–269CrossRefGoogle Scholar
  6. Brown MV, Bowman JP (2001) A molecular phylogenetic survey of sea-ice microbial communities (SIMCO). FEMS Microbiol Ecol 35:267–275CrossRefPubMedGoogle Scholar
  7. Greer G (1983) Psychrotrophic Brocothrix thermospacta bacteriophages isolated from beef. Appl Environ Microbiol 46:245–251PubMedGoogle Scholar
  8. Grossi SM, Kottmeier ST, Sullivan CW (1984) Sea ice microbial communities. III. Seasonal abundance of microalgae and associated bacteria, McMurdo Sound, Antarctica. Microb Ecol 10:231–242Google Scholar
  9. Grossmann S, Dieckmann G (1994) Bacterial standing stock, activity, and carbon production during formation and growth of sea ice in the Weddell Sea, Antarctica. Appl Environ Microbiol 60:2746–2753Google Scholar
  10. Guixa-Boixereu N, Vaque D, Gaso JM, Sanchez-Camara J, Pedros-Alio C (2002) Viral distribution and activity in Antarctic waters. Deep-Sea Res 49:827–845Google Scholar
  11. Helmke E, Weyland H (1995) Bacteria in sea ice and underlying water of the eastern Weddell Sea in midwinter. Mar Ecol Prog Ser 117:269–287Google Scholar
  12. Hofer JS, Sommaruga R (2001) Seasonal dynamics of viruses in an alpine lake: importance of filamentous forms. Aquat Microb Ecol 26:1–11Google Scholar
  13. Jiang SC, Kellogg CA, Paul JH (1998) Characterization of marine temperate phage-host systems isolated from Mamala Bay, Oahu, Hawaii. Appl Environ Microbiol 648:535–542Google Scholar
  14. Kepner RL Jr, Wharton RA Jr, Suttle CA (1998) Viruses in Antarctic lakes. Limnol Oceanogr 43:1754–1761PubMedGoogle Scholar
  15. Kottmeier ST; Sullivan CW (1987) Late winter primary production and bacterial production in sea ice and seawater west of the Antarctic Peninsula. Mar Ecol Prog Ser 36:287–298Google Scholar
  16. Kottmeier ST, Grossi SM, Sullivan CW (1987) Sea ice microbial communities. VIII. Bacterial production in annual sea ice of McMurdo Sound, Antarctica. Mar Ecol Prog Ser 35:175–186Google Scholar
  17. Maidak BL, et al (1999) A new version of the RDP (Ribosomal Database Project). Nucleic Acids Res 27:171–173PubMedGoogle Scholar
  18. Maranger R, Bird DF, Juniper SK (1994) Viral and bacterial dynamics in Arctic sea ice during the spring algal bloom near Resolute, NWT, Canada. Mar Ecol Prog Ser 111:121–127Google Scholar
  19. Marchant H, Davidson A, Wright S, Glazebrook J (2000) The distribution and abundance of viruses in the Southern Ocean during spring. Antarct Sci 12:414–417Google Scholar
  20. Moebus K (1991) Preliminary observations on the concentration of marine bacteriophages in the water around Helgoland. Helgol Wiss Meeresunters 45:411–422Google Scholar
  21. Moebus K, Nattkemper H (1983) Taxonomic investigations of bacteriophage sensitive bacteria isolated from marine waters. Helgol Meeresunters 36:357–373Google Scholar
  22. Morita RY (1975) Psychrophilic bacteria. Bact Rev 39:144–167Google Scholar
  23. Noble RT, Fuhrman JA (2000) Rapid virus production and removal as measured with fluorescently labeled viruses as tracers. Appl Environ Microbiol 66:3790–3797CrossRefPubMedGoogle Scholar
  24. Olsen RH (1967) Isolation and growth of psychrophilic bacteriophage. Appl Microbiol 15:198PubMedGoogle Scholar
  25. Oren A, Bratbak G, Heldal M (1997) Occurrence of virus-like particles in the Dead Sea. Extremophiles 1:143–149CrossRefPubMedGoogle Scholar
  26. Pinhassi J, Zweifel UL, Hagstrom A (1997) Dominant marine bacterioplankton species found among colony-forming bacteria. Appl Environ Microbiol 63:3359–3366PubMedGoogle Scholar
  27. Priddle J, Leakey R, Archer S, Murphy E (1996) Eukaryotic microbiota in the surface waters and sea ice of the Southern Ocean: aspects of physiology, ecology and biodiversity in a 'two-phase' system. Biodivers Cons 5:1473–1504Google Scholar
  28. Proctor LM, Fuhrman JA (1990) Viral mortality of marine cyanobacteria and bacteria. Nature 343:60–62CrossRefGoogle Scholar
  29. Rainey FA, Ward-Rainey N, Kroppenstedt RM, Stackebrandt E (1996) The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092PubMedGoogle Scholar
  30. Smith RE, Clement P (1990) Heterotrophic activity and bacterial productivity in assemblages of microbes from sea ice in the high Arctic. Polar Biol 10:351–357Google Scholar
  31. Smith DC, Steward GF, Azam F, Hollibaugh JT (1992) Virus and bacteria abundance in the Drake Passage during January and August 1991. Antarct J US 27:125–127Google Scholar
  32. Staley JT, Gosink JJ (1999) Poles apart: biodiversity and biogeography of sea ice bacteria. Annu Rev Microbiol 53:189–215PubMedGoogle Scholar
  33. Steward FG, Smith DC, Azam F (1996) Abundance and production of bacteria and viruses in the Bering and Chukchi Seas. Mar Ecol Prog Ser 131:287–300Google Scholar
  34. Suttle CA, Chan AM, Cottrell MT (1990) Infection of phytoplankton by viruses and reduction of primary productivity. Nature 347:467–469CrossRefGoogle Scholar
  35. Tan TL (1997) Biolog metabolic fingerprints for clustering marine oligotrophic bacteria from polar regions In: Insam H, Rangers A (eds) Microbial communities: function versus structural approaches. Springer, Berlin Heidelberg New York, pp 161–170Google Scholar
  36. Thomas DN, Dieckmann GS (2002) Antarctic sea ice habitat for extremophiles. Science 5555:641–644CrossRefGoogle Scholar
  37. Truong LV, Tuyen H, Helmke E, Binh LT, Schweder T (2001) Cloning and characterization of two cold-adapted pectate lyases from the marine Antarctic bacterium Pseudoalteromonas haloplanktis strain ANT/505. Extremophiles 5:35–44CrossRefPubMedGoogle Scholar
  38. Vincent WF, Gibson JAE, Pienitz R, Villeneuve V (2000) Ice shelf microbial ecosystems in the high Arctic and implications for life on snowball earth. Naturwissenschaften 87:137–141PubMedGoogle Scholar
  39. Wichels A, Biel SS, Gelderblom HR, Brinkhoff T, Muyzer G, Schütt C (1998) Bacteriophage diversity in the North Sea. Appl Environ Microbiol 64:4128–4133PubMedGoogle Scholar
  40. Wommack KE, Colwell RR (2000) Virioplankton: viruses in aquatic ecosystems. Microbiol Mol Biol Rev 64:69–114CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Michael Borriss
    • 1
  • Elisabeth Helmke
    • 2
  • Renate Hanschke
    • 3
  • Thomas Schweder
    • 1
    • 3
    Email author
  1. 1.Institut für Marine BiotechnologieGreifswaldGermany
  2. 2.Alfred-Wegener-Institut für Polar- und MeeresforschungBremerhavenGermany
  3. 3.Institut für MikrobiologieErnst-Moritz-Arndt-Universität GreifswaldGreifswaldGermany

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