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Extremophiles

, Volume 7, Issue 5, pp 353–360 | Cite as

Effect of temperature and pressure on the proteolytic specificity of the recombinant 20S proteasome from Methanococcus jannaschii

  • Rob J. Frankenberg
  • Maria Andersson
  • Douglas S. ClarkEmail author
Original Paper
  • 82 Downloads

Abstract

The hydrolytic specificity of the recombinant 20S proteasome from the deep-sea thermophile Methanococcus jannaschii was evaluated toward oxidized insulin B-chain across a range of temperatures (35°, 55°, 75°, and 90°C) and hydrostatic pressures (1, 250, 500, and 1,000 atm). Of the four temperatures considered, the same maximum overall hydrolysis rate was observed at both 55° and 75°C, which are much lower than the T opt of 116°C previously observed for a small amide substrate (Michels and Clark 1997). At 35°C the rates of cleavage were highest at the carboxyl side of glutamine and leucine, whereas at the three higher temperatures, the most rapid cleavages occurred after leucine and glutamic acid residues. The distribution of proteolytic fragments and the cleavage sequence also varied between the lowest and higher temperatures. Application of hydrostatic pressure did not increase proteasome activity, as observed previously for the amide substrate (Michels and Clark 1997), but instead significantly reduced the overall conversion of the polypeptide substrate. Overall cleavage patterns observed for the recombinant M. jannaschii proteasome were similar to those reported previously for Thermoplasma acidophilum (Akopian et al. 1997) and human proteasomes (Dick et al. 1991), indicating that proteasome specificity has been conserved despite significant environmental diversity.

Keywords

High pressure Hydrolytic specificity Methanococcus jannaschii Thermophilic proteasome 

Notes

Acknowledgments

This research was supported by the Bayer Corparation, UCSF/NIGMS (R25GM6847-02), NSF (BES-0224733), the Schlumberger Fellowship of DSC, and the Kyowa Hakko Kogyo Co. The authors would like to thank Michael Ru for his assistance in preparing the manuscript, and the following for their helpful contributions: David Altreuter, Tina Hsu, Richard Carrillo, and Peter Zwickl.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Rob J. Frankenberg
    • 1
  • Maria Andersson
    • 2
  • Douglas S. Clark
    • 3
    Email author
  1. 1.Bayer Health CareBerkeleyUSA
  2. 2.Department of BiotechnologyUniversity of LundLundSweden
  3. 3.Department of Chemical EngineeringUniversity of CaliforniaBerkeleyUSA

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