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Extremophiles

, Volume 7, Issue 4, pp 283–290 | Cite as

A novel lineage of sulfate-reducing microorganisms: Thermodesulfobiaceae fam. nov., Thermodesulfobium narugense, gen. nov., sp. nov., a new thermophilic isolate from a hot spring

  • Koji Mori
  • Hongik Kim
  • Takeshi Kakegawa
  • Satoshi Hanada
Original Paper

Abstract

A novel type of a sulfate-reducing microorganism, represented by strain Na82T, was isolated from a hot spring in Narugo, Japan. The isolate was a moderate thermophilic autotroph that was able to grow on H2/CO2 by sulfate respiration. The isolate could grow with nitrate in place of sulfate, and possessed menaquinone-7 and menaquinone-7(H2) as respiratory quinones. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain Na82T was a member of the domain Bacteria and distant from any known bacteria, as well as from other sulfate-reducing bacteria (sequence similarities less than 80%). The phylogenetic analysis of the dsrAB gene (alpha and beta subunits of dissimilatory sulfite reductase) sequence also suggested that strain Na82T was not closely related to other sulfate reducers. On the basis of the phenotypic and phylogenetic data, a new taxon is established for the isolate. We proposed the name Thermodesulfobium narugense gen. nov., sp. nov. with strain Na82T (=DSM 14796T=JCM 11510T) as the type strain. Furthermore, a new family, Thermodesulfobiaceae fam. nov., is proposed for the genus.

Keywords

Anaerobe Hot spring Sulfate-reducing bacterium Thermodesulfobiaceae fam. nov. Thermodesulfobium narugense gen. nov., sp. nov. Thermophile 

Notes

Acknowledgments

We thank Xian-Ying Meng (National Institute of Advanced Industrial Science and Technology) for electron microscopy. The research was supported by the Ministry of Education, Science and Technology (MEST), Japan, through Special Coordination Fund "Archaean Park Project" (International Research Project on Interaction Between Sub-Vent Biosphere and Geo-Environments).

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Koji Mori
    • 1
  • Hongik Kim
    • 1
  • Takeshi Kakegawa
    • 2
  • Satoshi Hanada
    • 1
  1. 1.Research Institute of Biological ResourcesNational Institute of Advanced Industrial Science and Technology (AIST)Tsukuba 305-8566Japan
  2. 2.Tohoku UniversitySendaiJapan

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