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Extremophiles

, Volume 6, Issue 5, pp 391–395 | Cite as

Revision of the taxonomic position of the xylanolytic Bacillus sp. MIR32 reidentified as Bacillus halodurans and plasmid-mediated transformation of B. halodurans

  • Alejandra M. Martínez
  • Osvaldo D. Delgado
  • Javier D. Breccia
  • Mario D. Baigorí
  • Faustino Siñeriz
Original Paper

Abstract.

Bacillus sp. MIR32 has been isolated using xylan as the only carbon source, and one of its xylanolytic enzymes has been extensively studied. Biochemical analysis first related this strain to Bacillus amyloliquefaciens, but further studies based on a comparison of 16S rDNA sequences, G+C content, and DNA–DNA hybridization showed that strain MIR32 should be classified as a member of the species Bacillus halodurans. This change is also supported by the typical phenotype observed and by the results of PCR amplification directed toward spacers in rDNA and tDNA genes, which were assayed and compared with those of B. halodurans DSM 497T. Although among alkaliphilic bacilli competence development has not been experimentally demonstrated, in this work both B. halodurans MIR32 and DSM 497T were transformed according to a simple procedure developed in our laboratory, reaching 102–103 stable transformants per microgram of plasmid DNA.

B. halodurans MIR32 Molecular characterization Transformation 16S rDNA sequence analysis DNA–DNA hybridization 16S–23S Intergenic spacer region 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Alejandra M. Martínez
    • 1
  • Osvaldo D. Delgado
    • 1
  • Javier D. Breccia
    • 1
  • Mario D. Baigorí
    • 1
  • Faustino Siñeriz
    • 1
  1. 1.PROIMI Planta Piloto de Procesos Industriales Microbiológicos, Av. Belgrano y Pasaje Caseros, 4000 San Miguel de Tucumán, Tucumán, Argentina

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