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Dietary fatty acids and the time elapsed from their intake are related to their composition in rat submandibular gland and salivary flow rates

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Abstract

Objectives

The aim of this study was to analyze the influence of dietary fatty acids (FAs) and the time elapsed from their intake on FA tissue profile of rat submandibular gland (SG) and on its salivary flow rate (SFR). Do dietary FAs depending on the intake time modify their profile in SG and consequently the SFR?

Materials and methods

Thirty-six adult male Wistar rats were fed on control diet (corn oil, CD, 18:2 n-6 FA) for 7 days and then divided into CD and two groups with replacement of corn oil by olive (OD, 18:1 n-9 FA) or chia (ChD, 18:3 n-3 FA) oils (1 and 30 day intake). Submandibular ducts were canalized to collect saliva for 20 min (μL/min). SG were examined (optical/electron microscopy; ImageJ 1.48 software).

Results

SFR values were 6.18 ± 0.34 (CD1), 6.04 ± 0.31 (OD1), and 6.00 ± 0.50 (ChD1) (p > 0.05). At 30-day intake, higher SFR values in ChD (7.82 ± 0.7) with respect to CD (4.68 ± 0.44; p < 0.001) and OD (6.08 ± 0.2; p = 0.038) were found. ChD30 showed a higher serous acinous area percentage than CD30 and OD30, whereas mucous acinous density was greater in CD30 than in OD30 and ChD30 (p < 0.05). α-Linolenic (ALA) and eicosapentaenoic and docosahexaenoic acid levels were only detected in SG of ChD30, while arachidonic acid was lower in this group as compared with CD30 and OD30 (p < 0.05).

Conclusions

SG FA composition and its SFR appear to be modulated by dietary FAs and the time elapsed from their consumption. SFR is highest with n-3 ALA-rich ChD at 30-day intake.

Clinical relevance

Diet could contribute to improve secretory dysfunctions.

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Funding

The work was supported by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and the Secretaría de Ciencia y Tecnología, Universidad Nacional de Córdoba, Argentina.

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Authors and Affiliations

  1. Facultad de Ciencias de la Salud, Universidad Adventista de Plata, 25 de mayo 99, 3103, Libertador San Martín, Entre Ríos, Argentina

    Jorge Escandriolo Nackauzi

  2. Facultad de Odontología, Universidad Nacional de Córdoba, Córdoba, Argentina

    Jorge Escandriolo Nackauzi & Raquel Gallará

  3. Instituto de Investigaciones en Ciencias de la Salud, Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, FCM, Universidad Nacional de Córdoba, Córdoba, Argentina

    Gastón Repossi & Adriana Actis

  4. Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral and Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, Santa Fe, Argentina

    Claudio Bernal

Authors
  1. Jorge Escandriolo Nackauzi
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  2. Gastón Repossi
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  3. Claudio Bernal
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  4. Adriana Actis
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  5. Raquel Gallará
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Jorge Dario Escandriolo Nackauzi, Raquel Gallará, Gastón Repossi, Claudio Bernal, and Adriana Actis. The original manuscript was written by Jorge Dario Escandriolo Nackauzi, and all authors worked on it in reviewing and editing, and they read and approved the final version.

Corresponding author

Correspondence to Jorge Escandriolo Nackauzi.

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Conflict of interest

Jorge Dario Escandriolo Nackauzi has received a research grant from “Secretaría de Ciencia y Tecnología”, Universidad Nacional de Córdoba. Gastón Repossi declares that he has no conflict of interest. Claudio Bernal declares that he has no conflict of interest. Adriana Actis declares that he has no conflict of interest. Raquel Gallará declares that he has no conflict of interest.

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Actis AB and Gallará RV are co-last authors.

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Escandriolo Nackauzi, J., Repossi, G., Bernal, C. et al. Dietary fatty acids and the time elapsed from their intake are related to their composition in rat submandibular gland and salivary flow rates. Clin Oral Invest 24, 4123–4131 (2020). https://doi.org/10.1007/s00784-020-03285-6

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