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Effects of ionizing radiation on woven bone: influence on the osteocyte lacunar network, collagen maturation, and microarchitecture

  • Eduardo Moura Mendes
  • Milena Suemi Irie
  • Gustavo Davi Rabelo
  • Juliana Simeão Borges
  • Paula Dechichi
  • Rafael Soares Diniz
  • Priscilla Barbosa Ferreira SoaresEmail author
Original Article
  • 38 Downloads

Abstract

Objectives

Evaluate the effects of ionizing radiation on microarchitecture, the osteocyte lacunar network, and collagen maturity in a bone repair site.

Materials and methods

Bone defects were created on tibias of 20 New Zealand rabbits. After 2 weeks, the animals were randomly divided into (n = 10) NoIr (nonirradiated group) and Ir (irradiated group). In the Ir, the animals received single-dose irradiation of 30 Gy on the tibia and were euthanized after 2 weeks. Bone microarchitecture parameters were analyzed by using micro-CT, and the osteocyte lacunar network, bone matrix, and collagen maturation by histomorphometric analysis. The data were analyzed using unpaired Student’s t test (α = 0.05).

Results

Trabecular thickness in Ir was lower than that in NoIr (P = 0.028). No difference was found for bone volume fraction and bone area. Lacunae filled with osteocytes were more numerous (P < 0.0001) in NoIr (2.6 ± 0.6) than in Ir (1.97 ± 0.53). Empty lacunae were more prevalent (P < 0.003) in Ir (0.14 ± 0.10) than in NoIr (0.1 ± 0.1). The mean osteocyte lacunae size was higher (P < 0.01) in Ir (15.4 ± 4.41) than in NoIr (12.7 ± 3.7). Picrosirius red analysis showed more (P < 0.05) mature collagen in NoIr (29.0 ± 5.3) than in Ir (23.4 ± 4.5). Immature collagen quantification revealed no difference between groups.

Conclusions

Ionizing radiation compromised bone formation and an impairment in bone repair in irradiated woven bone was observed.

Clinical relevance

Before radiotherapy, patients usually need surgical intervention, which may be better performed, if clinicians understand the repair process in irradiated bone, using novel approaches for treating these individuals.

Keywords

Ionizing radiation Bone repair Osteocyte X-ray microtomography Collagen 

Notes

Acknowledgments

The authors are grateful to the Department of Clinical Oncology at Federal University of Triângulo Mineiro (UFTM), MG, Brazil.

Funding information

This study was financed in part by Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Informed consent

For this type of study, formal consent is not required.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Eduardo Moura Mendes
    • 1
  • Milena Suemi Irie
    • 1
  • Gustavo Davi Rabelo
    • 2
  • Juliana Simeão Borges
    • 1
  • Paula Dechichi
    • 3
  • Rafael Soares Diniz
    • 4
  • Priscilla Barbosa Ferreira Soares
    • 1
    Email author
  1. 1.Department of Periodontology and Implantology, School of DentistryFederal University of UberlândiaUberlândiaBrazil
  2. 2.Dentistry DepartmentFederal University of Santa CatarinaFlorianópolisBrazil
  3. 3.Institute of Biomedical SciencesFederal University of UberlândiaUberlândiaBrazil
  4. 4.Department of Postgraduate Program in DentistryCEUMA UniversitySão LuisBrazil

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