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Effects of argon plasma treatment on the osteoconductivity of bone grafting materials

  • Luigi CanulloEmail author
  • Tullio Genova
  • Mia Rakic
  • Anton Sculean
  • Richard Miron
  • Maurizio Muzzi
  • Stefano Carossa
  • Federico Mussano
Original Article

Abstract

Background

The osteoconductive properties of bone grafting materials represent one area of research for the management of bony defects found in the fields of periodontology and oral surgery. From a physico-chemical aspect, the wettability of the graft has been demonstrated to be one of the most important factors for new bone formation. It is also well-known that argon plasma treatment (PAT) and ultraviolet irradiation (UV) may increase the surface wettability and, consequently, improve the regenerative potential of the bone grafts. Therefore, the aim of the present in vitro study was to evaluate the effect of PAT and UV treatment on the osteoconductive potential of various bone grafts.

Materials and methods

The following four frequently used bone grafts were selected for this study: synthetic hydroxyapatite (Mg-HA), biphasic calcium phosphate (BCP), cancellous and cortical xenogenic bone matrices (CaBM, CoBM). Sixty-six serially numbered disks 10 mm in diameter were used for each graft material and randomly assigned to the following three groups: test 1 (PAT), test 2 (UV), and control (no treatment). Six samples underwent topographic analysis using SEM pre- and post-treatments to evaluate changes in surface topography/characteristics. Additionally, cell adhesion and cell proliferation were evaluated at 2 and 72 h respectively following incubation in a three-dimensional culture system utilizing a bioreactor. Furthermore, the effects of PAT and UV on immune cells were assessed by measuring the viability of human macrophages at 24 h.

Results

The topographic analysis showed different initial morphologies of the commercial biomaterials (e.g., Mg-HA and BCP showed flat morphology; BM samples were extremely porous with high roughness). The surface analysis following experimental treatments did not demonstrate topographical difference when compared with controls. Investigation of cells demonstrated that PAT treatment significantly increased cell adhesion of all 4 evaluated bone substitutes, whereas UV failed to show any statistically significant differences. The viability test revealed no differences in terms of macrophage adhesion on any of the tested surfaces.

Conclusion

Within their limitations, the present results suggest that treatment of various bone grafting materials with PAT appears to enhance the osteoconductivity of bone substitutes in the early stage by improving osteoblast adhesion without concomitantly affecting macrophage viability.

Clinical relevance

Treatment of bone grafts with PAT appears to result in faster osseointegration of the bone grafting materials and may thus favorably influence bone regeneration.

Keywords

Bone graft Plasma of argon Bio-activation Osseointegration Osteoconductivity 

Notes

Acknowledgments

Authors highly appreciated the skills and commitment of Dr. Audrenn Gautier in the supervision of the study and Dr. Henry Canullo for the scientific support.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The study is an in vitro study. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Private practiceRomeItaly
  2. 2.CIR Dental School, Department of Surgical SciencesUniversity of TurinTurinItaly
  3. 3.Department of PeriodontologyNantes UniversityNantesFrance
  4. 4.Department of Periodontology, School of Dental MedicineUniversity of BernBernSwitzerland
  5. 5.Department of ScienceUniversity Roma TreRomeItaly
  6. 6.Laboratorio Interdipartimentale di Microscopia Elettronica (LIME)University Roma TreRomeItaly

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