Alveolar crest contour changes after guided bone regeneration using different biomaterials: an experimental in vivo investigation

  • R. Di Raimondo
  • J. Sanz-Esporrín
  • R. Plá
  • I. Sanz-Martín
  • F. Luengo
  • F. Vignoletti
  • J. Nuñez
  • Mariano SanzEmail author
Original Article



To evaluate the changes in alveolar contour after guided bone regeneration (GBR) with two different combinations of biomaterials in dehiscence defects around implants.

Material and methods

Chronic alveolar ridge defects were created bilaterally in the mandible of eight Beagle dogs. Once implants were placed, three treatment groups were randomly allocated to each peri-implant dehiscence defect: (i) test group received a bone substitute composed of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) covered by a cross-linked collagen membrane, (ii) positive control group with placement of deproteinized bovine bone mineral (DBBM) plus a porcine natural collagen membrane, and (iii) a negative control with no treatment. Two healing periods (8 and 16 weeks) were evaluated. Dental casts were optically scanned, the obtained files were uploaded into an image analysis software and superimposed to evaluate the linear changes.


In both healing periods, the gains in linear contours were higher in the test group and at the intermediate level (3 mm below the gingival margin). While at 8 weeks, no significant differences were found between the groups; at 16 weeks, the test and positive control groups demonstrated significant gains in contour compared with negative control.


GBR using different biomaterials significantly increased the buccal contours of the alveolar crest when used at dehiscence defects around dental implants.

Clinical relevance

Particulate highly porous synthetic bone substitute and a cross-linked collagen membrane demonstrated similar outcomes in terms of contour augmentation when compared to bovine xenograft (DBBM) and a collagen membrane.


Guided bone regeneration Synthetic bone graft Collagen membrane Dental implant Animal model Prophilometric changes 

MeSH Terms

Bone Regeneration Calcium Phosphates Membranes Biocompatible Materials Dental Implants Animal Model Alveolar Bone Loss 



The authors acknowledge professor Ui-Won Jung for the active involvement in the surgical procedure. We also thank the veterinary doctors, Maria Carmen Calles-Vázquez and Elena Abellán, as well as the staff from the Minimally Invasive Surgery Centre, Cáceres, Spain, who so effectively took care of the experimental animals used in this investigation.

Funding information

This work was partially supported through a research contract between the University Complutense of Madrid and Dentium Implants, Suwon (Korea). Support was also obtained from the ETEP (Etiology and therapeutics in Periodontal Diseases) Research Group at the Faculty of Odontology, University Complutense of Madrid (Spain).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article contains data from an experimental study with animals performed at the Experimental Surgical Department of the Minimally Invasive Surgery Centre in Cáceres (Spain) after receiving approval from the Regional Ethics Committee for Animal Research (CCMIJU Reference 011/15). All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Informed consent

For this type of study, formal consent is not required.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Section of Graduate PeriodontologyUniversity ComplutenseMadridSpain
  2. 2.ETEP (Etiology and Therapy of Periodontal Diseases) Research GroupUniversity ComplutenseMadridSpain
  3. 3.Facultad de OdontologíaPlaza Ramón y Cajal s/n (Ciudad Universitaria)MadridSpain

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