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Correlation of magnetic resonance imaging grades with cytokine levels of synovial fluid of patients with temporomandibular joint disorders: a cross-sectional study

  • Mu-Chen Yang
  • Ding-Han Wang
  • Hung-Ta Wu
  • Wan-Chun Li
  • Tsai-Yu Chang
  • Wen-Liang LoEmail author
  • Ming-Lun HsuEmail author
Original Article
  • 15 Downloads

Abstract

Objectives

Magnetic resonance imaging (MRI) is a standardized method for assisting joint diagnosis. To validate the reliability of different imaging-based grading systems, this study examined (1) the associations between grading systems for osseous change, joint effusion, and the Wilkes classification of temporomandibular joint (TMJ) disorders and (2) the correlation between cytokines in synovial fluid and imaging-based joint scores.

Materials and methods

Twenty-seven patients, who routinely received numeric rating scale (NRS) and MRI assessment before TMJ arthrocentesis, were enrolled. Each joint was evaluated through the grading criteria for severity of osseous change and joint effusion by blinded observers using MRI. ImageJ was employed for classifying joint effusion. Joint synovial fluid, collected through arthrocentesis, was examined for cytokine expression by using a Luminex multiplex assay. All data were analyzed using the Pearson correlation analysis.

Results

The Wilkes classification was strongly correlated with osseous change scores, but not with joint effusion scores. Joint effusion scores significantly correlated with NRS scores, but not with the Wilkes classification and osseous change scores. Compared with osseous change scores, joint effusion scores had a higher correlation with the levels of inflammatory cytokines (interleukin (IL)-8 and soluble IL-6 receptor (sIL-6R)) and with anti-inflammatory cytokines (soluble tumor necrosis factor receptors I and II (sTNF-RI/II)).

Conclusions

In patients with TMJ disorders, MRI grades are strongly correlated with NRS scores and levels of cytokines (IL-8, sIL-6R, and sTNF-RI/II) in the synovial fluid.

Clinical relevance

Joint effusion scoring can be a reliable and valid indicator for pathological assessment of TMJ disorders.

Keywords

Temporomandibular joint Magnetic resonance imaging Cytokines Joint effusion 

Notes

Funding

This research was supported by a grant from the Ministry of Science and Technology, Taiwan (MOST105-2314-B-010-031-MY2).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and national research committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

784_2019_2817_MOESM1_ESM.pdf (159 kb)
Figure S1 No significant correlation between expression of cytokines and the Wilkes classification. TNF-α, IL-6, IFN-γ, MMP 3, MMP 12, MMP 13, IL-1β, IL-1ra, sIL-1RII, IL-4, IL-10, and IL-13 exhibited no significant correlations with the Wilkes classification. Tumor necrosis factor-α (TNF-α); Interleukin (IL); Interferon (IFN)-γ; Matrix metalloproteinase (MMP); IL-1 receptor antagonist (IL-1ra); Soluble IL-1 receptor II (sIL-1RII). (PDF 158 kb)
784_2019_2817_MOESM2_ESM.pdf (158 kb)
Figure S2 No significant correlation between expression of cytokines and the osseous change score. TNF-α, IL-6, IFN-γ, MMP 3, MMP 12, MMP 13, IL-1β, IL-1ra, sIL-1RII, IL-4, IL-10, and IL-13exhibited no significant correlations with the osseous change score. (PDF 157 kb)
784_2019_2817_MOESM3_ESM.pdf (159 kb)
Figure S3 No significant correlation between expression of cytokines and joint effusion score. TNF-α, IL-6, IFN-γ, MMP 3, MMP 12, MMP 13, IL-1β, IL-1ra, sIL-1RII, IL-4, IL-10, and IL-13 exhibited no significant correlations with the joint effusion score. (PDF 158 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Dentistry, School of DentistryNational Yang-Ming UniversityTaipeiTaiwan
  2. 2.Department of RadiologyTaipei Veterans General HospitalTaipeiTaiwan
  3. 3.Institute of Oral Biology, School of DentistryNational Yang-Ming UniversityTaipeiTaiwan
  4. 4.Cancer Progression CenterNational Yang-Ming UniversityTaipeiTaiwan
  5. 5.Division of Oral and Maxillofacial Surgery, Department of StomatologyTaipei Veterans General HospitalTaipeiTaiwan

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