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Chemical analysis of in vivo–irradiated dentine of head and neck cancer patients by ATR-FTIR and Raman spectroscopy

  • Rafael Resende de Miranda
  • Anielle Christine Almeida Silva
  • Noelio Oliveira Dantas
  • Carlos José Soares
  • Veridiana Resende Novais
Original Article
  • 24 Downloads

Abstract

Objectives

To evaluate the effect of in vivo radiotherapy on the chemical properties of human dentine by Fourier-transform infrared spectroscopy (FTIR) and Raman analysis.

Materials and methods

Chemical composition was evaluated comparing control and irradiated group (n = 8). Irradiated teeth were obtained from radiotherapy patients subjected to fractionated X-ray radiation of 1.8 Gy daily totaling 72 Gy. The teeth were sectioned according to the type of dentine (crown or root dentine), obtaining 3-mm dentine cervical slices. The analyzed parameters by FTIR and Raman spectroscopies were mineral/matrix ratio (M:M), carbonate/mineral ratio (C:M), amide I/amide III ratio, and amide I/CH2 ratio. Raman also calculated the phosphate and carbonate crystallinity.

Results

FTIR revealed that M:M had a decrease in both factors (p = 0.008; p = 0.043, respectively) and root dentine showed a lower C:M in the irradiated group (p = 0.003). Raman revealed a higher phosphate crystallinity and a lower carbonate crystallinity in crown dentine of irradiated group (p = 0.021; p = 0.039). For amide I/amide III, the irradiated showed a lower ratio when compared to the control group (FTIR p = 0.002; Raman p = 0.017). For amide I/CH2, the root dentine showed a higher ratio than the crown dentine in both methods (p < 0.001).

Conclusions

Radiotherapy altered the chemical composition of human dentine. The exchange of phosphate-carbonate ions in the hydroxyapatite and higher concentration of organic components was found after radiotherapy.

Clinical relevance

The increased risk of radiation-related caries in patients undergoing head and neck radiotherapy is due not only to salivary, dietary, and microbiological changes but also to changes in tooth chemical composition.

Keywords

Dentine Fourier-transform infrared spectroscopy Head and neck cancer Radiotherapy Raman spectroscopy 

Notes

Acknowledgements

The authors thank FAPEMIG, CNPq and CAPES (Brazil) for the financial support. The authors are also grateful to the Laboratory of New Insulating Materials and Semiconductors (LNMIS) at Institute of Physics, Federal University of Uberlândia, Minas Gerais, Brazil.

Funding

The study was supported by FAPEMIG, CNPq and CAPES for the granting of scholarship and resource for the purchase of consumer material.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study. Additional informed consent was obtained from all individual participants for whom identifying information is included in this article.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Operative Dentistry and Dental Materials, School of DentistryFederal University of UberlândiaUberlândiaBrazil
  2. 2.Laboratory of New Nanostructured and Functional Materials, Institute of PhysicsFederal University of AlagoasMaceióBrazil

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