Systemic administration of curcumin or piperine enhances the periodontal repair: a preliminary study in rats

  • Morgana R. Guimaraes-StabiliEmail author
  • Sabrina Garcia de Aquino
  • Fabiana de Almeida Curylofo
  • Camilla Olga Tasso
  • Fernanda Regina Godoy Rocha
  • Marcell Costa de Medeiros
  • José Paulo de PizzolJr
  • Paulo Sérgio Cerri
  • Giuseppe Alexandre Romito
  • Carlos RossaJr
Original Article



Studies have documented the anti-inflammatory effects of spices, which may be related to treatment of chronic diseases. The purpose of this study was to evaluate the influence of curcumin and piperine and their association on experimental periodontal repair in rats.

Materials and methods

Periodontitis was induced via the installation of a ligature around the first molar. After 15 days, the ligatures were removed, and the rats were separated into groups (12 animals per group): (i) curcumin, (ii) piperine, (iii) curcumin+piperine, (iv) corn oil vehicle, and (v) control group (animals had ligature-induced periodontitis but were not treated). The compounds were administered daily, for 15 days by oral gavage. Animals were euthanized at 5 and 15 days, and hemimaxillae and gingival tissues were harvested. Bone repair was assessed by μCT (microcomputer tomography). Histological sections were stained with hematoxylin/eosin (H/E) for the assessment of cellular infiltrate or picrosirius red for quantification of collagen content, and subjected to immunohistochemistry for detecting NF-ĸB. Gingival tissues were used to evaluate levels of TGF-β and IL-10 (ELISA).


Curcumin and piperine increased the TGF-β level, significantly improved the collagen repair, and decreased the cellularity and activation of NF-ĸB in the periodontal tissues, but only curcumin caused a significant increase in early bone repair.


Curcumin and piperine promoted a substantive effect on tissue repair; however, there was not synergistic effect of compounds administered in combination.

Clinical relevance

Curcumin and piperine stimulates the tissue repair and may be potential candidates for the treatment of periodontal disease.


Periodontal disease Curcumin Piperine Repair Rats 



The work was supported by FAPESP (São Paulo Research Foundation) 2010/19660-2 and 2010/20091-2.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All the experimental protocols were approved by the Ethical Committee for Animal Use (CEUA) of the School of Dentistry at Araraquara – UNESP and performed in accordance with the guidelines from the Brazilian College for Animal Experimentation (COBEA). The reported data conforms to the ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Morgana R. Guimaraes-Stabili
    • 1
    Email author
  • Sabrina Garcia de Aquino
    • 2
  • Fabiana de Almeida Curylofo
    • 1
  • Camilla Olga Tasso
    • 1
  • Fernanda Regina Godoy Rocha
    • 1
  • Marcell Costa de Medeiros
    • 1
  • José Paulo de PizzolJr
    • 3
  • Paulo Sérgio Cerri
    • 3
  • Giuseppe Alexandre Romito
    • 4
  • Carlos RossaJr
    • 1
  1. 1.Department of Diagnosis and SurgerySchool of Dentistry at Araraquara - Univ Estadual Paulista (UNESP)AraraquaraBrazil
  2. 2.Department of Clinical and Social Dentistry- Health Science CenterFederal University of ParaíbaJoão PessoaBrazil
  3. 3.Department of Histology and EmbriologySchool of Dentistry at Araraquara, UNESPAraraquaraBrazil
  4. 4.Department of StomatologySchool of Dentistry-University of Sao Paulo (USP)Sao PauloBrazil

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