Mineral density changes in bovine carious dentin after treatment with bioactive dental cements: a comparative micro-CT study
- 153 Downloads
To evaluate the potential of conventional glass ionomer cement (GIC), Biodentine™, MTA, and Portland cement to induce mineral density changes in carious dentin compared to zinc oxide eugenol control cement (ZOE).
Materials and methods
Fifty blocks of bovine root dentin were prepared and a biofilm model using ATCC strains of S.mutans, S.sobrinus, and L.casei was used to promote artificial dentin lesions. After demineralization, the blocks were randomly divided into the five cement groups. Half of the surface of each specimen received the tested material and the other half was covered with wax (control). Samples were stored in phosphate buffered saline solution for 30 days and after that were scanned in a micro-CT with standardized parameters. Dentin mineral density changes were calculated using differences in plot profiles of the exposed and control carious dentin. Friedman’s test, followed by Wilcoxon signed-rank test was used with 5% significance.
Mean ΔZ values for the cements were 48.63 ± 19.09 for the control (ZOE), 63.31 ± 32.59 for Biodentine™, 114.63 ± 72.92 for GIC, 109.56 ± 66.28 for MTA, and 106.88 ± 66.02 for Portland cement. All cements showed a statistically significant increase in ΔZ values compared to the control, but Biodentine™ values were statistically significantly lower compared to GIC and the other calcium silicate cements.
Tested materials present potential to induce mineral density changes in carious bovine dentin. MTA, Portland, and GIC showed higher bioactivity potential than Biodentine™.
Based on minimally invasive concept, materials with remineralization potential can be used to preserve diseased but still repairable dental tissue.
KeywordsDentin caries Bioactive cements Micro-CT MTA Glass ionomer
The authors are grateful to the Department of Pediatric Dentistry and Orthodontics in Federal University of Rio de Janeiro (UFRJ) and Laboratory for Nuclear Instrumentation (UFRJ).
The work was supported by the Department of Pediatric Dentistry and Orthodontics and Laboratory for Nuclear Instrumentation of Federal University of Rio de Janeiro. This study was supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Pesquisa Científica (CNPq), and Fundação Carlos Chagas de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
For this type of study, formal consent is not required.
- 3.Hamama HH, Yiu CK, Burrow MF (2015) Caries management: a journey between Black’s principals and minimally invasive concepts. Int J Dent Oral Sci 2(8):120–125Google Scholar
- 4.Watson TF, Atmeh AR, Sajini S, Cook RJ, Festy F (2014) Present and future of glass-ionomers and calcium-silicate cements as bioactive materials in dentistry: biophotonics-based interfacial analyses in health and disease. Dent Mater 30(1):50–61. https://doi.org/10.1016/j.dental.2013.08.202 CrossRefPubMedPubMedCentralGoogle Scholar
- 7.Liu Y, Li N, Qi Y, Niu LN, Elshafiy S, Mao J, Breschi L, Pashley DH, Tay FR (2011) The use of sodium trimetaphosphate as a biomimetic analog of matrix phosphoproteins for remineralization of artificial caries-like dentin. Dent Mater 27(5):465–477. https://doi.org/10.1016/j.dental.2011.01.008 CrossRefPubMedPubMedCentralGoogle Scholar
- 10.Okamoto M, Takahashi Y, Komichi S, Ali M, Yoneda N, Ishimoto T, Nakano T, Hayashi M (2018) Novel evaluation method of dentin repair by direct pulp capping using high-resolution micro-computed tomography. Clin Oral Investig. https://doi.org/10.1007/s00784-018-2374-5
- 19.Pires PM, Santos TP, Fonseca-Goncalves A, Pithon MM, Lopes RT, Neves AA (2018) Mineral density in carious dentine after treatment with calcium silicates and polyacrylic acid based cements. Int Endod J. https://doi.org/10.1111/iej.12941