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Biodentine and MTA modulate immunoinflammatory response favoring bone formation in sealing of furcation perforations in rat molars

  • Tiago Silva da Fonseca
  • Guilherme F. Silva
  • Juliane M. Guerreiro-Tanomaru
  • Mateus Machado Delfino
  • Estela Sasso-Cerri
  • Mário Tanomaru-Filho
  • Paulo Sérgio Cerri
Original Article
  • 199 Downloads

Abstract

Objectives

Evaluate the tissue reaction of periodontium subjacent to furcation perforations in rat molars sealed with Biodentine or mineral trioxide aggregate (MTA).

Materials and methods

The pulp chamber floor of right upper first molars of 60 rats was perforated and filled with Biodentine, MTA, or cotton pellet (sham); the left first molars were used as control. After 7, 15, 30, and 60 days, maxillary fragments were processed for paraffin-embedding. The periodontal space (PS), volume density of inflammatory cells (VvIC) and fibroblasts (VvFb), number of osteoclasts, and collagen content were obtained. Interleukin-6 (IL-6) and osterix (osteoblast marker) were detected by immunohistochemistry. The data were submitted to ANOVA and Tukey’s test (p ≤ 0.05).

Results

At 7 days, high values in VvIC, IL-6-immunolabeled cells, and osteoclasts were accompanied by reduced collagen content in enlarged PS of experimental groups. At all periods, VvIC, number of osteoclasts and IL-6, and PS were higher in sham than in Biodentine and MTA (p < 0.0001). From 7 to 60 days, significant reduction in VvIC, IL-6 immunoexpression, and osteoclasts was accompanied by significant increase in VvFb, osteoblasts, and collagen in Biodentine and MTA groups. At 60 days, significant differences in VvIC, PS, IL-6, osteoclasts, and osteoblasts were not found between Biodentine and MTA. Significant differences in the osteoclast number were not observed among Biodentine, MTA, and control groups while osteoblasts number was higher in Biodentine and MTA groups.

Conclusions

Despite the initial inflammatory reaction and bone resorption, the sealing of furcation perforations with Biodentine and MTA favors the repair of periodontal tissues.

Clinical relevance

Biodentine and MTA exhibit potential as repair material in the treatment of furcation perforations.

Keywords

Calcium silicate cement Bone remodeling Periodontium repair Immunohistochemistry 

Notes

Acknowledgements

The authors thank Mr. Luis Antônio Potenza and Mr. Pedro Sérgio Simões for kind help and technical assistance. We thank the Amazonas Research Foundation – FAPEAM (Brazil) for the fellowship grant awarded to Tiago Silva da Fonseca (Proc. No. 117/2014) and Coordination for the Improvement of Higher Education Personnel – CAPES (Brazil) for the fellowship grant to Guilherme Ferreira da Silva and Mateus Machado Delfino.

Funding

This research was supported by São Paulo Research Foundation (FAPESP, Brazil) and National Council for Scientific and Technological Development (CNPq, Brazil).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The animal care and the experimental procedures were conducted in accordance with Brazilian national law on animal use. The Ethical Committee for Animal Research of Dental School of Araraquara (São Paulo State University - UNESP, Brazil) approved the protocol regarding the animal use and experimental procedures.

Informed consent

For this type of study, formal consent is not required.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Tiago Silva da Fonseca
    • 1
  • Guilherme F. Silva
    • 2
  • Juliane M. Guerreiro-Tanomaru
    • 1
  • Mateus Machado Delfino
    • 3
  • Estela Sasso-Cerri
    • 3
  • Mário Tanomaru-Filho
    • 1
  • Paulo Sérgio Cerri
    • 3
  1. 1.UNESP- Univ. Estadual Paulista, Dental School, Department of Restorative DentistryAraraquaraBrazil
  2. 2.School of Dentistry (USC-Bauru), Pro-Rectory of Research and Post GraduationUniversidade Sagrado CoraçãoBauruBrazil
  3. 3.UNESP - Univ Estadual Paulista, Dental School, Department of Morphology, Laboratory of Histology and EmbryologyAraraquaraBrazil

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