Biodentine and MTA modulate immunoinflammatory response favoring bone formation in sealing of furcation perforations in rat molars
- 155 Downloads
Evaluate the tissue reaction of periodontium subjacent to furcation perforations in rat molars sealed with Biodentine or mineral trioxide aggregate (MTA).
Materials and methods
The pulp chamber floor of right upper first molars of 60 rats was perforated and filled with Biodentine, MTA, or cotton pellet (sham); the left first molars were used as control. After 7, 15, 30, and 60 days, maxillary fragments were processed for paraffin-embedding. The periodontal space (PS), volume density of inflammatory cells (VvIC) and fibroblasts (VvFb), number of osteoclasts, and collagen content were obtained. Interleukin-6 (IL-6) and osterix (osteoblast marker) were detected by immunohistochemistry. The data were submitted to ANOVA and Tukey’s test (p ≤ 0.05).
At 7 days, high values in VvIC, IL-6-immunolabeled cells, and osteoclasts were accompanied by reduced collagen content in enlarged PS of experimental groups. At all periods, VvIC, number of osteoclasts and IL-6, and PS were higher in sham than in Biodentine and MTA (p < 0.0001). From 7 to 60 days, significant reduction in VvIC, IL-6 immunoexpression, and osteoclasts was accompanied by significant increase in VvFb, osteoblasts, and collagen in Biodentine and MTA groups. At 60 days, significant differences in VvIC, PS, IL-6, osteoclasts, and osteoblasts were not found between Biodentine and MTA. Significant differences in the osteoclast number were not observed among Biodentine, MTA, and control groups while osteoblasts number was higher in Biodentine and MTA groups.
Despite the initial inflammatory reaction and bone resorption, the sealing of furcation perforations with Biodentine and MTA favors the repair of periodontal tissues.
Biodentine and MTA exhibit potential as repair material in the treatment of furcation perforations.
KeywordsCalcium silicate cement Bone remodeling Periodontium repair Immunohistochemistry
The authors thank Mr. Luis Antônio Potenza and Mr. Pedro Sérgio Simões for kind help and technical assistance. We thank the Amazonas Research Foundation – FAPEAM (Brazil) for the fellowship grant awarded to Tiago Silva da Fonseca (Proc. No. 117/2014) and Coordination for the Improvement of Higher Education Personnel – CAPES (Brazil) for the fellowship grant to Guilherme Ferreira da Silva and Mateus Machado Delfino.
This research was supported by São Paulo Research Foundation (FAPESP, Brazil) and National Council for Scientific and Technological Development (CNPq, Brazil).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The animal care and the experimental procedures were conducted in accordance with Brazilian national law on animal use. The Ethical Committee for Animal Research of Dental School of Araraquara (São Paulo State University - UNESP, Brazil) approved the protocol regarding the animal use and experimental procedures.
For this type of study, formal consent is not required.
- 3.Silva LAB, Pieroni KAMG, Nelson-Filho P, Silva RAB, Hernandéz-Gatón P, Lucisano MP, Paula-Silva FWG, de Queiroz AM (2017) Furcation perforation: periradicular tissue response to biodentine as a repair material by histopathologic and indirect immunofluorescence analyses. J Endod 43:1137–1142. https://doi.org/10.1016/j.joen.2017.02.001 CrossRefPubMedGoogle Scholar
- 6.da Fonseca TS, da Silva GF, Tanomaru-Filho M, Sasso-Cerri E, Guerreiro-Tanomaru JM, Cerri PS (2016) In vivo evaluation of the inflammatory response and IL-6 immunoexpression promoted by Biodentine and MTA Angelus. Int Endod J 49:145–153. https://doi.org/10.1111/iej.12435 CrossRefPubMedGoogle Scholar
- 7.Rodrigues EM, Gomes-Cornélio AL, Soares-Costa A, Salles LP, Velayutham M, Rossa-Junior C, Guerreiro-Tanomaru JM, Tanomaru-Filho M (2017) An assessment of the overexpression of BMP-2 in transfected human osteoblast cells stimulated by mineral trioxide aggregate and Biodentine. Int Endod J 50:e9–e18. https://doi.org/10.1111/iej.12745 CrossRefPubMedGoogle Scholar
- 9.De Rossi A, Silva LAB, Gatón-Hernández P, Sousa-Neto MD, Nelson-Filho P, Silva RA, de Queiroz AM (2014) Comparison of pulpal responses to pulpotomy and pulp capping with Biodentine and mineral trioxide aggregate in dogs. J Endod 40:1362–1369. https://doi.org/10.1016/j.joen.2014.02.006 CrossRefPubMedGoogle Scholar
- 10.Bosso-Martelo R, Guerreiro-Tanomaru JM, Viapiana R, Berbert FL, Duarte MA, Tanomaru-Filho M (2016) Physicochemical properties of calcium silicate cements associated with microparticulate and nanoparticulate radiopacifiers. Clin Oral Investig 20:83–90. https://doi.org/10.1007/s00784-015-1483-7 CrossRefPubMedGoogle Scholar
- 11.Silva GF, Guerreiro-Tanomaru JM, da Fonseca TS, Bernardi MIB, Sasso-Cerri E, Tanomaru-Filho M, Cerri PS (2017) Zirconium oxide and niobium oxide used as radiopacifiers in a calcium silicate-based material stimulate fibroblast proliferation and collagen formation. Int Endod J 50:e95–e108. https://doi.org/10.1111/iej.12789 CrossRefPubMedGoogle Scholar
- 12.Silva GF, Tanomaru-Filho M, Bernardi MIB, Guerreiro-Tanomaru JM, Cerri PS (2015) Niobium pentoxide as radiopacifying agent of calcium silicate-based material: evaluation of physicochemical and biological properties. Clin Oral Investig 19:2015–2025. https://doi.org/10.1007/s00784-015-1412-9 CrossRefPubMedGoogle Scholar
- 14.Grazziotin-Soares R, Nekoofar MH, Davies TE, Bafail A, Alhaddar E, Hübler R, Busato AL, Dummer PM (2014) Effect of bismuth oxide on white mineral trioxide aggregate: chemical characterization and physical properties. Int Endod J 47:520–533. https://doi.org/10.1111/iej.12181 CrossRefPubMedGoogle Scholar
- 19.Cuadros-Fernández C, Lorente Rodríguez AI, Sáez-Martínez S, García-Binimelis J, About I, Mercadé M (2016) Short-term treatment outcome of pulpotomies in primary molars using mineral trioxide aggregate and Biodentine: a randomized clinical trial. Clin Oral Investig 20:1639–1645. https://doi.org/10.1007/s00784-015-1656-4 CrossRefPubMedGoogle Scholar
- 21.Nowicka A, Lipski M, Parafiniuk M, Sporniak-Tutak K, Lichota D, Kosierkiewicz A, Kaczmarek W, Buczkowska-Radlińska J (2013) Response of human dental pulp capped with Biodentine and mineral trioxide aggregate. J Endod 39:743–747. https://doi.org/10.1016/j.joen.2013.01.005 CrossRefPubMedGoogle Scholar
- 23.Camilleri J, Grech L, Galea K, Keir D, Fenech M, Formosa L, Damidot D, Mallia B (2014) Porosity and root dentine to material interface assessment of calcium silicate-based root-end filling materials. Clin Oral Investig 18:1437–1446. https://doi.org/10.1007/s00784-013-1124-y CrossRefPubMedGoogle Scholar
- 24.Gomes-Cornélio AL, Rodrigues EM, Salles LP, Mestieri LB, Faria G, Guerreiro-Tanomaru JM, Tanomaru-Filho M (2017) Bioactivity of MTA Plus, Biodentine and an experimental calcium silicate-based cement on human osteoblast-like cells. Int Endod J 50:39–47. https://doi.org/10.1111/iej.12589 CrossRefPubMedGoogle Scholar
- 28.de Oliveira PA, de Pizzol-Júnior JP, Longhini R, Sasso-Cerri E, Cerri PS (2017) Cimetidine reduces interleukin-6, matrix metalloproteinases-1 and -9 immunoexpression in the gingival mucosa of rat molars with induced periodontal disease. J Periodontol 88:100–111. https://doi.org/10.1902/jop.2016.160132 CrossRefPubMedGoogle Scholar
- 37.Silva GF, Bosso R, Ferino RV, Tanomaru-Filho M, Bernardi MI, Guerreiro-Tanomaru JM, Cerri PS (2014) Microparticulated and nanoparticulated zirconium oxide added to calcium silicate cement: evaluation of physicochemical and biological properties. J Biomed Mater Res A 102:4336–4345. https://doi.org/10.1002/jbm.a.35099 CrossRefPubMedGoogle Scholar
- 39.Torres FFE, Bosso-Martelo R, Espir CG, Cirelli JA, Guerreiro-Tanomaru JM, Tanomaru-Filho M (2017) Evaluation of physicochemical properties of root-end filling materials using conventional and Micro-CT tests. J Appl Oral Sci 25:374–380. https://doi.org/10.1590/1678-7757-2016-0454 CrossRefPubMedPubMedCentralGoogle Scholar
- 41.Williams DF (2008) On the mechanisms of biocompatibility. Biomaterials 29:2941–2953. https://doi.org/10.1016/j.biomaterials.2008.04.023 CrossRefPubMedGoogle Scholar
- 45.Nakashima T, Hayashi M, Fukunaga T, Kurata K, Oh-Hora M, Feng JQ, Bonewald LF, Kodama T, Wutz A, Wagner EF, Penninger JM, Takayanagi H (2011) Evidence for osteocyte regulation of bone homeostasis through RANKL expression. Nat Med 17:1231–1234. https://doi.org/10.1038/nm.2452 CrossRefPubMedGoogle Scholar
- 56.Michel A, Erber R, Frese C, Gehrig H, Saure D, Mente J (2017) In vitro evaluation of different dental materials used for the treatment of extensive cervical root defects using human periodontal cells. Clin Oral Investig 21:753–761. https://doi.org/10.1007/s00784-016-1830-3 CrossRefPubMedGoogle Scholar