Comparative differentiation analysis of distinct oral tissue-derived cells in response to osteogenic stimulation
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Mixed cell populations from oral tissues may be superior to pure stem cells for regenerative approaches. Therefore, the aim of the present study was to explore the osteogenic potential of mixed cells derived from oral connective tissues compared to alveolar osteoblasts.
Materials and methods
Primary cells were isolated from the alveolar bone, periodontal ligament and gingiva. Following characterization by colony formation, growth capacity and flow cytometry, all cells were subjected to osteogenic differentiation induction and screened for a large panel of osteogenic markers using western blots, qPCR arrays, and matrix mineralization and alkaline phosphatase quantification.
Non-induced mixed cells from gingiva showed higher colony formation efficiency but decreased proliferation compared to non-induced periodontal mixed cells, while both entities revealed similar surface markers tested in this setup. Following osteogenic induction, all cell populations individually expressed receptors with distinctively activated downstream effectors. Gene expression of induced periodontal mixed cells was similar to alveolar osteoblasts, but was differently modulated in gingival mixed cells. The latter failed to achieve osteogenic differentiation in terms of matrix mineralization and alkaline phosphatase activity, which was well observed in periodontal mixed cells and osteoblasts.
Mixed cells from periodontal ligament but not from gingiva feature an inherent osteogenic capacity in vitro. From these results, it can be concluded that periodontal cells do not require further stem cell enrichment in order to qualify for bone regeneration.
Our data contribute to the development of novel cell-based therapies using mixed cells from the periodontal ligament in regenerative periodontics.
KeywordsOsteoblasts (MeSH-ID D010006) Periodontal ligament (MeSH-ID D010513) Gingiva (MeSH-ID D005881) Cells (MeSH-ID D002477) Osteogenesis (MeSH-ID D010012)
The authors are grateful to Anja Kaufmann for her excellent technical assistance.
The corresponding author is indebted to the Baden Württemberg Stiftung for the financial support of this research project by the ‘Eliteprogramme for Postdocs’.
Compliance with ethical standards
Conflicts of interest
The authors declare that they have no competing interests.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the Committee of Ethics of the Medical Faculty of the Albert Ludwigs University Freiburg, Germany, and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed consent was obtained from all individual participants included in the study.
- 1.Hu L, Liu Y and Wang S (2017) Stem cell-based tooth and periodontal regeneration. Oral Dis . doi: 10.1111/odi.12703Google Scholar
- 8.Fawzy El-Sayed KM, Dorfer CE Gingival mesenchymal stem/progenitor cells: a unique tissue engineering gem. Stem Cells Int 2016, 2016:7154327–7154316. https://doi.org/10.1155/2016/7154327
- 22.Kundrotas G (2012) Surface markers distinguishing mesenchymal stem cells from fibroblasts. Acta Med Lituan 19:75–79Google Scholar
- 24.Pisciotta A, Carnevale G, Meloni S, Riccio M, De Biasi S, Gibellini L, Ferrari A, Bruzzesi G, De Pol A (2015) Human dental pulp stem cells (hDPSCs): isolation, enrichment and comparative differentiation of two sub-populations. BMC Dev Biol 15:14. https://doi.org/10.1186/s12861-015-0065-x CrossRefGoogle Scholar
- 33.Beederman MLJ, Nan G, Wang J, Liu X, Yin L, Li R, Shui W, Zhang H, Kim SH, Zhang W, Zhang J, Kong Y, Denduluri S, Rogers MR, Pratt A, Haydon RC, Luu HH, Angeles J, Shi LL, He TC (2013) BMP signaling in mesenchymal stem cell differentiation and bone formation. J Biomed Sci Eng 6:32–52CrossRefGoogle Scholar
- 34.Ishibashi O, Ikegame M, Takizawa F, Yoshizawa T, Moksed MA, Iizawa F, Mera H, Matsuda A, Kawashima H (2010) Endoglin is involved in BMP-2-induced osteogenic differentiation of periodontal ligament cells through a pathway independent of Smad-1/5/8 phosphorylation. J Cell Physiol 222:465–473. https://doi.org/10.1002/jcp.21968 CrossRefGoogle Scholar
- 37.Matsubara T, Suardita K, Ishii M, Sugiyama M, Igarashi A, Oda R, Nishimura M, Saito M, Nakagawa K, Yamanaka K, Miyazaki K, Shimizu M, Bhawal UK, Tsuji K, Nakamura K, Kato Y (2005) Alveolar bone marrow as a cell source for regenerative medicine: differences between alveolar and iliac bone marrow stromal cells. J Bone Miner Res 20:399–409. https://doi.org/10.1359/jbmr.041117 CrossRefGoogle Scholar
- 38.Paldi A (2013) Effects of the in vitro manipulation of stem cells: epigenetic mechanisms as mediators of induced metabolic fluctuations. Epigenomics 5:429–437. doi: 10.2217/epi.13.35Google Scholar