Bioactive glass-ceramic bone repair associated or not with autogenous bone: a study of organic bone matrix organization in a rabbit critical-sized calvarial model
- 114 Downloads
The aim of the study was to analyze bone matrix (BMX) organization after bone grafting and repair using a new bioactive glass-ceramic (Biosilicate®) associated or not with particulate autogenous bone graft.
Material and methods
Thirty rabbits underwent surgical bilateral parietal defects and divided into groups according to the materials used: (C) control—blood clot, (BG) particulate autogenous bone, (BS) bioactive glass-ceramic, and BG + BS. After 7, 14, and 30 days post-surgery, a fragment of each specimen was fixed in − 80 °C liquid nitrogen for zymographic evaluation, while the remaining was fixed in 10% formalin for histological birefringence analysis.
The results of this study demonstrated that matrix organization in experimental groups was significantly improved compared to C considering collagenous organization. Zymographic analysis revealed pro-MMP-2, pro-MMP-9, and active (a)-MMP-2 in all groups, showing gradual decrease of total gelatinolytic activity during the periods. At day 7, BG presented more prominent gelatinolytic activity for pro-MMP-2 and 9 and a-MMP-2, when compared to the other groups. In addition, at day 7, a 53% activation ratio (active form/[active form + latent form]) was evident in C group, 33% in BS group, and 31% in BG group.
In general, BS allowed the production of a BMX similar to BG, with organized collagen deposition and MMP-2 and MMP-9 disponibility, permitting satisfactory bone remodeling at the late period.
The evaluation of new bone substitute, with favorable biological properties, opens the possibility for its use as a viable and efficient alternative to autologous bone graft.
KeywordsBiomaterials Bone repair Picrosirius red Vitroceramic Zymography
The authors are grateful to Maira Cristina Rondina Couto for histology assistance.
This work was supported by São Paulo Research Foundation (FAPESP/SP), grant numbers 2008/11485-8; 2009/17294-1.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures performed in studies involving animals were in accordance with the ethical standards of the Ethical Committee for Animal Research of Sagrado Coração University (protocol # 110/09), and the Brazilian College of Animal Experimentation (COBEA) guidelines for the care and use of laboratory animals.
For this type of study, formal consent is not required.
- 2.Eweida AM, Nabawi AS, Abouarab M, Kayed M, Habashi E, Etaby A, Khalil MR, Shawky MS, Kneser U, Horch RE, Nagy N, Marei MK (2014) Enhancing mandibular bone regeneration and perfusion via axial vascularization of scaffolds. Clin Oral Invest 18:1671–1678. https://doi.org/10.1007/s00784-013-1143-8 CrossRefGoogle Scholar
- 5.Peitl O, Zanotto ED, La Torre GP. Hench LL (1997) Patent WO/1997/041079Google Scholar
- 6.Zanotto ED, Ravagnani C, Peitl OF, Panzeri H, Guimarães LEH (2004) Fundação Universidade Federal de São Carlos, Universidade de São Paulo. Process and compositions for preparing particulate, bioactive or resorbable biosilicates for use in the treatment of oral ailments. Int. C. C03C10/00, WO2004/074199Google Scholar
- 7.Peitl O, Zanotto ED, Serbena FC, Hench LL (2012) Acta Biomater 8(1):321–332. Compositional and microstructural design of highly bioactive P2O5-Na2O-CaO-SiO2 glass-ceramics CrossRefGoogle Scholar
- 9.Tsigkou O, Jones JR, Polak JM, Stevens MM (2009) Differentiation of fetal osteoblasts and formation of mineralized bone nodules by 45S5 Bioglass conditioned medium in the absence of osteogenic supplements. Biomaterials 30(21):3542–3550. https://doi.org/10.1016/j.biomaterials.2009.03.019 CrossRefGoogle Scholar
- 10.Granito RN, Rennó AC, Ravagnani C, Bossini PS, Mochiuti D, Jorgetti V, Driusso P, Peitl O, Zanotto ED, Parizotto NA, Oishi J (2011) In vivo biological performance of a novel highly bioactive glass-ceramic (Biosilicate®): a biomechanical and histomorphometric study in rat tibial defects. J Biomed Mater Res B Appl Biomater 97((1):139–147. https://doi.org/10.1002/jbm.b.31795 CrossRefGoogle Scholar
- 11.Roriz VM, Rosa AL, Peitl O, Zanotto ED, Panzeri H, de Oliveira PT (2010) Efficacy of a bioactive glass-ceramic (Biosilicate) in the maintenance of alveolar ridges and in osseointegration of titanium implants. Clin Oral Implants Res 21(2):148–155. https://doi.org/10.1111/j.1600-0501.2009.01812.x CrossRefGoogle Scholar
- 13.van Houdt CI, Tim CR, Crovace MC, Zanotto ED, Peitl O, Ulrich DJ, Jansen JA, Parizotto NA, Renno AC, van den Beucken JJ (2015) Bone regeneration and gene expression in bone defects under healthy and osteoporotic bone conditions using two commercially available bone graft substitutes Biomed Mater. 8;10(3):035003. doi: https://doi.org/10.1088/1748-6041/10/3/035003
- 20.Nyman JS, Lynch CC, Perrien DS, Thiolloy S, O'Quinn EC, Patil CA, Bi X, Pharr GM, Mahadevan-Jansen A, Mundy GR (2011) Differential effects between the loss of MMP-2 and MMP-9 on structural and tissue-level properties of bone. J Bone Miner Res 26(6):1252–1260. https://doi.org/10.1002/jbmr.326 CrossRefGoogle Scholar
- 23.Zhou Z, Apte SS, Soininen R, Cao R, Baaklini GY, Rauser RW, Wang J, Cao Y, Tryggvason K (2000) Impaired endochondral ossification and angiogenesis in mice deficient in membrane-type matrix metalloproteinase I. Proc Natl Acad Sci U S A 97(8):4052–4057. https://doi.org/10.1073/pnas.060037197 CrossRefGoogle Scholar
- 28.Garavello-Freitas I, Baranauskas V, Joazeiro PP, Padovani CR, Dal Pai-Silva M, da Cruz-Hofling MA (2003) Low-power laser irradiation improves histomorphometrical parameters and bone matrix organization during tibia wound healing in rats. J Photochem Photobiol B 70(2):81–89. https://doi.org/10.1016/S1011-1344(03)00058-7 CrossRefGoogle Scholar
- 31.Osorio C, Cavalla F, Paula-Lima A, Dıaz-Araya G, Vernal R, Ahumada P, Gamonal J, Herandez M (2015) H2O2 activates matrix metalloproteinases through the nuclear factor kappa B pathway and Ca2+signals in human periodontal fibroblasts. J Periodontal Res 50(6):798–806. https://doi.org/10.1111/jre.12267 CrossRefGoogle Scholar
- 32.Cavalla F, Osorio C, Paredes R, Valenzuela MA, García-Sesnich J, Sorsa T, Tervahartiala T, Hernández M (2015) Matrix metalloproteinases regulate extracellular levels of SDF-1/CXCL12, IL-6 and VEGF in hydrogen peroxide-stimulated human periodontal ligament fibroblasts. Cytokine 73(1):114–121. https://doi.org/10.1016/j.cyto.2015.02.001 CrossRefGoogle Scholar
- 34.Martins CH, Carvalho TC, Souza MG, Ravagnani C, Peitl O, Zanotto ED, Panzeri H, Casemiro LA (2011) Assessment of antimicrobial effect of Biosilicate against anaerobic, microaerophilic and facultative anaerobic microorganisms. J Mater Sci Mater Med 22(6):1439–1446. https://doi.org/10.1007/s10856-011-4330-7 CrossRefGoogle Scholar
- 37.Vieira AE, Repeke CE, Ferreira Junior SB, Colavite PM, Biguetti CC, Oliveira RC, Asssis GF, Taga R, Trombone APF, Garlet GP (2015) Intramembranous bone healing process subsequent to tooth extraction in mice: micro-computed tomography, histomorphometric and molecular characterization. PLoS One 10(5): e012802 doi: https://doi.org/10.1371/journal.pone.0128021, e0128021
- 38.Lu M, Rabie AB (2006) Matrix metalloproteinase-9 regulates graft bone resorption. Angle Orthod 76(4):598–604Google Scholar