Dentin sealing and antibacterial effects of silver-doped bioactive glass/mesoporous silica nanocomposite: an in vitro study

  • Jae-Hyun Jung
  • Dong-Hyun Kim
  • Kyung-Hyeon Yoo
  • Seog-Young Yoon
  • Yeon Kim
  • Moon-Kyoung Bae
  • Jin Chung
  • Ching-Chang Ko
  • Yong Hoon Kwon
  • Yong-Il Kim
Original Article
  • 63 Downloads

Abstract

Objectives

To synthesize a silver-doped bioactive glass/mesoporous silica nanoparticle (Ag-BGN@MSN), as well as to investigate its effects on dentinal tubule occlusion, microtensile bond strength (MTBS), and antibacterial activity.

Materials and methods

Ag-BGN@MSN was synthesized using a modified “quick alkali-mediated sol-gel” method. Demineralized tooth disc models were made and divided into four groups; the following treatments were then applied: group 1—no treatment, group 2—bioglass, group 3—MSN, group 4—Ag-BGN@MSN. Next, four discs were selected from each group and soaked into 6 wt% citric acid to test acid-resistant stability. Dentinal tubule occlusion, as well as the occlusion ratio, was observed using field-emission scanning electron microscopy. The MTBS was also measured to evaluate the desensitizing effect of the treatments. Cytotoxicity was examined using the MTT assay. Antibacterial activity was detected against Lactobacillus casei, and ion dissolution was evaluated using inductively coupled plasma optical emission spectrometry.

Results

Ag-BGN@MSN effectively occluded the dentinal tubule and formed a membrane-like layer. After the acid challenge, Ag-BGN@MSN had the highest rate of dentinal tubule occlusion. There were no significant differences in MTBS among the four groups (P > 0.05). All concentrations of Ag-BGN@MSN used had a relative cell viability above 72%.

Conclusions

Ag-BGN@MSN was successfully fabricated using a modified sol-gel method. The Ag-BGN@MSN biocomposite effectively occluded dentinal with acid-resistant stability, did not decrease bond strength in self-etch adhesive system, had low cytotoxicity, and antibacterial effect.

Clininal relevance

Dentinal tubule sealing induced by Ag-BGN@MSN biocomposite with antibacterial effect is likely to increase long-term stability in DH.

Keywords

Bioactive glass Mesoporous silica Nanoparticle Antibacterial effect Remineralization Dentin hypersensitivity 

Notes

Compliance with ethical standards

Conflict of interest

YI Kim has received research grants from the National Research Foundation of Korea (NRF) for this study. All authors declare that they have no competing interest.

Ethical approval

The study was approved by the Ethics Committee of Pusan National University Dental Hospital (PNUDH-2016-033).

Informed consent

Informed consent was obtained from all individual participants included in the study.

Reference

  1. 1.
    Sanjay M, Vivek A, Bhoomika A (2010) Dentin hypersensitivity: recent trends in management. J Conserv Dent 13:218–224.  https://doi.org/10.4103/0972-0707.73385 CrossRefGoogle Scholar
  2. 2.
    Holland GR, Narhi MN, Addy M, Gangarosa L, Orchardson R (1997) Guidelines for the design and conduct of clinical trials on dentine hypersensitivity. J Clin Periodontol 24:808–813.  https://doi.org/10.1111/j.1600-051X.1997.tb01194.x CrossRefPubMedGoogle Scholar
  3. 3.
    Brannstrom M (1963) Dentin sensitivity and aspiration of odontoblasts. J Am Dent Assoc 66:366–370.  https://doi.org/10.14219/jada.archive.1963.0104 CrossRefPubMedGoogle Scholar
  4. 4.
    Rees JS, Jin U, Lam S, Kudanowska I, Vowles R (2003) The prevalence of dentine hypersensitivity in a hospital clinic population in Hong Kong. J Dent 31:453–461.  https://doi.org/10.1016/S0300-5712(03)00092-7 CrossRefPubMedGoogle Scholar
  5. 5.
    Flynn J, Galloway R, Orchardson R (1985) The incidence of ‘hypersensitive’ teeth in the West of Scotland. J Dent 13:230–236.  https://doi.org/10.1016/0300-5712(85)90004-1 CrossRefPubMedGoogle Scholar
  6. 6.
    Fischer C, Fischer RG, Wennberg A (1992) Prevalence and distribution of cervical dentine hypersensitivity in a population in Rio de Janeiro, Brazil. J Dent 20:272–276.  https://doi.org/10.1016/0300-5712(92)90043-C CrossRefPubMedGoogle Scholar
  7. 7.
    Wang Y, Que K, Lin L, Hu D, Li X (2012) Prevalence of dentine hypersensitivity in the general population in China. J Oral Rehabil 39:812–820.  https://doi.org/10.1111/j.1365-2842.2012.02334.x CrossRefPubMedGoogle Scholar
  8. 8.
    Liu HC, Lan WH, Hsieh CC (1998) Prevalence and distribution of cervical dentin hypersensitivity in a population in Taipei, Taiwan. J Endod 24:45–47.  https://doi.org/10.1016/S0099-2399(98)80213-6 CrossRefPubMedGoogle Scholar
  9. 9.
    Absi EG, Addy M, Adams D (1987) Dentine hypersensitivity: a study of the patency of dentinal tubules in sensitive and non-sensitive cervical dentine. J Clin Periodontol 14:280–284.  https://doi.org/10.1111/j.1600-051X.1987.tb01533.x CrossRefPubMedGoogle Scholar
  10. 10.
    Schmidlin PR, Sahrmann P (2013) Current management of dentin hypersensitivity. Clin Oral Investig 17:55–59.  https://doi.org/10.1007/s00784-012-0912-0 CrossRefGoogle Scholar
  11. 11.
    Pashley DH (1994) Dentin permeability and its role in the pathobiology of dentin sensitivity. Arch Oral Biol 39:73–80.  https://doi.org/10.1016/0003-9969(94)90191-0 CrossRefGoogle Scholar
  12. 12.
    Wang Z, Sa Y, Sauro S, Chen H, Xing W, Ma X (2010) Effect of desensitising toothpastes on dentinal tubule occlusion: a dentine permeability measurement and SEM in vitro study. J Dent 38:400–410.  https://doi.org/10.1016/j.jdent.2010.01.007 CrossRefPubMedGoogle Scholar
  13. 13.
    Canali GD, Rached RN, Mazur RF, Souza EM (2017) Effect of erosion/abrasion challenge on the dentin tubule occlusion using different desensitizing agents. Braz Dent J 28:216–224.  https://doi.org/10.1590/0103-6440201700811 CrossRefPubMedGoogle Scholar
  14. 14.
    Reis C, De-Deus G, Leal F, Azevedo E, Coutinho-Filho T, Paciornik S (2008) Strong effect on dentin after the use of high concentrations of citric acid: an assessment with co-site optical microscopy and ESEM. Dent Mater 24:1608–1615.  https://doi.org/10.1016/j.dental.2008.03.027 CrossRefPubMedGoogle Scholar
  15. 15.
    Wiegand A, Stock A, Attin R, Werner C, Attin T (2007) Impact of the acid flow rate on dentin erosion. J Dent 35:21–27.  https://doi.org/10.1016/j.jdent.2006.04.002 CrossRefPubMedGoogle Scholar
  16. 16.
    Pei D, Liu S, Huang C, Du X, Yang H, Wang WY (2013) Effect of pretreatment with calcium-containing desensitizer on the dentine bonding of mild self-etch adhesives. Eur J Oral Sci 121:204–210.  https://doi.org/10.1111/eos.12047 CrossRefPubMedGoogle Scholar
  17. 17.
    Tang F, Li L, Chen D (2012) Mesoporous silica nanoparticles: synthesis, biocompatibility and drug delivery. Adv Mater 24:1504–1534.  https://doi.org/10.1002/adma.201104763 CrossRefPubMedGoogle Scholar
  18. 18.
    Huang X, Young NP, Townley HE (2014) Characterisation and comparison of mesoporous silica particles for optimised drug delivery. Nanomater Nanotechnol 4:2–15.  https://doi.org/10.5772/58290 CrossRefGoogle Scholar
  19. 19.
    Tian L, Peng C, Shi Y, Guo X, Zhong B, Qi J et al (2014) Effect of mesoporous silica nanoparticles on dentinal tubule occlusion: an in vitro study using SEM and image analysis. Dent Mater J 33:125–132.  https://doi.org/10.4012/dmj.2013-215 CrossRefPubMedGoogle Scholar
  20. 20.
    Prabhakar AR, Paul MJ, Basappa N (2010) Comparative evaluation of the remineralizing effects and surface micro hardness of glass ionomer cements containing bioactive glass (S53P4): an in vitro study. Int J Clin Pediatr Dent 3:69–77.  https://doi.org/10.5005/jp-journals-10005-1057 CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Erol-Taygun M, Zheng K, Boccaccini AR (2013) Nanoscale bioactive glasses in medical applications. Int J Appl Glas Sci 4:136–148.  https://doi.org/10.1111/ijag.12029 CrossRefGoogle Scholar
  22. 22.
    Webster TJ, Ergun C, Doremus RH, Siegel RW, Bizios R (2000) Enhanced functions of osteoblasts on nanophase ceramics. Biomaterials 21:1803–1810.  https://doi.org/10.1016/S0142-9612(00)00075-2 CrossRefPubMedGoogle Scholar
  23. 23.
    Miguez-Pacheco V, Hench LL, Boccaccini AR (2015) Bioactive glasses beyond bone and teeth: emerging applications in contact with soft tissues. Acta Biomater 13:1–15.  https://doi.org/10.1016/j.actbio.2014.11.004 CrossRefPubMedGoogle Scholar
  24. 24.
    Hoppe A, Güldal NS, Boccaccini AR (2011) A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics. Biomaterials 32:2757–2774.  https://doi.org/10.1016/j.biomaterials.2011.01.004 CrossRefPubMedGoogle Scholar
  25. 25.
    Ruparelia JP, Chatterjee AK, Duttagupta SP, Mukherji S (2008) Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta Biomater 4:707–716.  https://doi.org/10.1016/j.actbio.2007.11.006 CrossRefPubMedGoogle Scholar
  26. 26.
    El-Kady AM, Ali AF, Rizk RA, Ahmed MM (2012) Synthesis, characterization and microbiological response of silver-doped bioactive glass nanoparticles. Ceram Int 38:177–188.  https://doi.org/10.1016/j.ceramint.2011.05.158 CrossRefGoogle Scholar
  27. 27.
    Shi X, Wang Y, Wei K, Ren L, Lai C (2008) Self-assembly of nanohydroxyapatite in mesoporous silica. J Mater Sci Mater Med 19:2933–2940.  https://doi.org/10.1007/s10856-008-3424-3 CrossRefPubMedGoogle Scholar
  28. 28.
    Xia W, Chang J (2007) Preparation and characterization of nano-bioactive-glasses (NBG) by a quick alkali-mediated sol–gel method. Mater Lett 61:3251–3253.  https://doi.org/10.1016/j.matlet.2006.11.048 CrossRefGoogle Scholar
  29. 29.
    Wang Y, Zhao Q, Han N, Bai L, Li J, Liu J, Che E, Hu L, Zhang Q, Jiang T, Wang S (2015) Mesoporous silica nanoparticles in drug delivery and biomedical applications. Nanomedicine 11:313–327.  https://doi.org/10.1016/j.nano.2014.09.014 CrossRefPubMedGoogle Scholar
  30. 30.
    Horcajada P, Rámila A, Boulahya K, González-Calbet J, Vallet-Regí M (2004) Bioactivity in ordered mesoporous materials. Solid State Sci 6:1295–1300.  https://doi.org/10.1016/j.solidstatesciences.2004.07.026 CrossRefGoogle Scholar
  31. 31.
    Lee BS, Chang CW, Chen WP, Lan WH, Lin CP (2005) In vitro study of dentin hypersensitivity treated by Nd:YAP laser and bioglass. Dent Mater 21:511–519.  https://doi.org/10.1016/j.dental.2004.08.002 CrossRefPubMedGoogle Scholar
  32. 32.
    Chiang YC, Chen HJ, Liu HC, Kang SH, Lee BS, Lin FH (2010) A novel mesoporous biomaterial for treating dentin hypersensitivity. J Dent Res 89:236–240.  https://doi.org/10.1177/0022034509357148 CrossRefPubMedGoogle Scholar
  33. 33.
    Yu J, Yang H, Li K, Lei J, Zhou L, Huang C (2016) A novel application of nanohydroxyapatite/mesoporous silica biocomposite on treating dentin hypersensitivity: an in vitro study. J Dent 50:21–29.  https://doi.org/10.1016/j.jdent.2016.04.005 CrossRefPubMedGoogle Scholar
  34. 34.
    Xu Z, Neoh KG, Kishen A (2008) Monitoring acid-demineralization of human dentine by electrochemical impedance spectroscopy (EIS). J Dent 36(12):1005–1012.  https://doi.org/10.1016/j.jdent.2008.08.007 CrossRefPubMedGoogle Scholar
  35. 35.
    Izquierdo-Barba I, Ruiz-González L, Doadrio JC, González-Calbet JM, Vallet-Regí M (2005) Tissue regeneration: a new property of mesoporous materials. Solid State Sci 7:983–989.  https://doi.org/10.1016/j.solidstatesciences.2005.04.003 CrossRefGoogle Scholar
  36. 36.
    Vollenweider M, Brunner TJ, Knecht S, Grass RN, Zehnder M, Imfeld T, Stark WJ (2007) Remineralization of human dentin using ultrafine bioactive glass particles. Acta Biomater 3:936–943.  https://doi.org/10.1016/j.actbio.2007.04.003 CrossRefPubMedGoogle Scholar
  37. 37.
    Vallet-Regí M, Izquierdo-Barba I, Rámila A, Pérez-Pariente J, Babonneau F, González-Calbet JM (2005) Phosphorous-doped MCM-41 as bioactive material. Solid State Sci 7:233–237.  https://doi.org/10.1016/j.solidstatesciences.2004.10.038 CrossRefGoogle Scholar
  38. 38.
    Adebayo OA, Burrow MF, Tyas MJ (2008) Dentine bonding after CPP-ACP paste treatment with and without conditioning. J Dent 36:1013–1024.  https://doi.org/10.1016/j.jdent.2008.08.011 CrossRefPubMedGoogle Scholar
  39. 39.
    Bergamin AC, Bridi EC, Amaral FL, Turssi CP, Basting RT, Aguiar FH, França FM (2016) Influence of an arginine-containing toothpaste on bond strength of different adhesive systems to eroded dentin. Gen Dent 64:67–73PubMedGoogle Scholar
  40. 40.
    Marshall SJ, Bayne SC, Baier R, Tomsia AP, Marshall GW (2010) A review of adhesion science. Dent Mater 26:11–16.  https://doi.org/10.1016/j.dental.2009.11.157 CrossRefGoogle Scholar
  41. 41.
    Yang H, Pei D, Liu S, Wang Y, Zhou L, Deng D (2013) Effect of a functional desensitizing paste containing 8% arginine and calcium carbonate on the microtensile bond strength of etch-and-rinse adhesives to human dentin. Am J Dent 26:137–142.  https://doi.org/10.1186/s40563-014-0024-y PubMedGoogle Scholar
  42. 42.
    Karpiński TM, Szkaradkiewicz AK (2013) Microbiology of dental caries. J Biol Earth Sci 3:21–24.  https://doi.org/10.1007/978-1-349-16547-6_3 Google Scholar
  43. 43.
    Jung WK, Koo HC, Kim KW, Shin S, Kim SH, Park YH (2008) Antibacterial activity and mechanism of action of the silver ion in Staphylococcus aureus and Escherichia coli. Appl Environ Microbiol 74:2171–2178.  https://doi.org/10.1128/AEM.02001-07 CrossRefPubMedPubMedCentralGoogle Scholar
  44. 44.
    Brown ML, Davis HB, Tufekci E, Crowe JJ, Covelle DA, Mitchell JC (2011) Ion release from a novel orthodontic resin bonding agent for the reduction and/or prevention of white spot lesions: an in vitro study. Angle Orthod 81:1014–1020.  https://doi.org/10.2319/120710-708.1 CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Orthodontics, Dental Research InstitutePusan National University Dental HospitalYangsanRepublic of Korea
  2. 2.R&D CenterUpex. Med Co., Ltd.AnyangRepublic of Korea
  3. 3.School of Materials Science and EngineeringPusan National UniversityBusanRepublic of Korea
  4. 4.Department of Oral Physiology, School of DentistryPusan National UniversityYangsanRepublic of Korea
  5. 5.Department of Oral Microbiology, School of DentistryPusan National UniversityYangsanRepublic of Korea
  6. 6.Department of OrthodonticsSchool of Dentistry, University of North Carolina at Chapel HillChapel HillUSA
  7. 7.Department of Dental MaterialsPusan National UniversityYangsanRepublic of Korea
  8. 8.Institute of Translational Dental Sciences, School of DentistryPusan National UniversityYangsanRepublic of Korea

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