Clinical Oral Investigations

, Volume 23, Issue 1, pp 161–167 | Cite as

Spectrophotometric analysis of discoloration and internal bleaching after use of different antibiotic pastes

  • Funda Fundaoğlu KüçükekenciEmail author
  • Fatih Çakici
  • Ahmet Serkan Küçükekenci
Original Article



To investigate teeth’s antibiotic-induced color differences after bleaching using two different techniques.

Materials and methods

One hundred twenty extracted maxillar human incisors were examined. The specimens were randomly divided into six groups, each receiving one of six antibiotic paste fillings: (1) triple antibiotic paste (TAP) with minocycline, (2) double antibiotic paste (DAP), (3) TAP with amoxicillin, (4) TAP with cefaclor, (5) TAP with doxycycline, and (6) no filling (control group). Spectrophotometric measurements were obtained at baseline and then during the first, second, and third weeks after paste placement. The specimens discolored by antibiotics pastes were randomly divided into two subgroups: (1) internal bleaching with hydrogen peroxide (H2O2) and (2) internal bleaching with H2O2 plus Nd-YAG laser irradiation. The ∆E value was calculated and analyzed using a two-way analysis of variance and post-hoc Tukey’s test (α = 0.05).


The ∆E for all groups showed color differences exceeding the perceptibility threshold (∆E ˃ 3.7) at all time points except in the control and DAP groups. Minocycline-induced TAP showed the most severe coronal discoloration (32.42). When the ∆E was examined, thermo/photo bleaching (22.01 ± 8.23) caused more bleaching than walking bleaching (19.73 ± 5.73) at every time point (P = 0.19). No group returned to the original color after bleaching (P < 0.05).


Except for DAP, all antibiotic pastes caused discoloration. Internal bleaching with Nd-YAG laser can be useful for bleaching/removing this discoloration.

Clinical relevance

For clinically successful final appearances, understanding the effects of bleaching procedures on antibiotic paste discoloration is important.


Antibiotics paste Bleaching Discoloration Nd-YAG laser Spectrophotometer 


Funding information

This research study was supported by Ordu University Scientific Research Committee (TU-1501).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in the studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

For this type of study, formal consent is not required.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Funda Fundaoğlu Küçükekenci
    • 1
    Email author
  • Fatih Çakici
    • 2
  • Ahmet Serkan Küçükekenci
    • 3
  1. 1.Ordu Public Oral Health HospitalOrduTurkey
  2. 2.Department of Endodontics, Faculty of DentistryOrdu UniversityOrduTurkey
  3. 3.Department of Prosthodontics, Faculty of DentistryOrdu UniversityOrduTurkey

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