Microstructure and chemical analysis of four calcium silicate-based cements in different environmental conditions
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The objective of this study was to analyze the microstructure and crystalline structures of ProRoot MTA, Biodentine, CEM Cement, and Retro MTA when exposed to phosphate-buffered saline, butyric acid, and blood.
Methods and materials
Mixed samples of ProRoot MTA, Biodentine, CEM Cement, and Retro MTA were exposed to either phosphate-buffered saline, butyric acid, or blood. Scanning electron microscope (SEM) and energy-dispersive X-ray spectroscopic (EDX) evaluations were conducted of specimens. X-ray diffraction (XRD) analysis was also performed for both hydrated and powder forms of evaluated calcium silicate cements.
The peak of tricalcium silicate and dicalcium silicate detected in all hydrated cements was smaller than that seen in their unhydrated powders. The peak of calcium hydroxide (Ca(OH)2) in blood- and acid-exposed ProRoot MTA, CEM Cement, and Retro MTA specimens were smaller than that of specimens exposed to PBS. The peak of Ca(OH)2 seen in Biodentine™ specimens exposed to blood was similar to that of PBS-exposed specimens. On the other hand, those exposed to acid exhibited smaller peaks of Ca(OH)2.
Exposure to blood or acidic pH decreased Ca(OH)2 crystalline formation in ProRoot MTA, CEM Cement and Retro MTA. However, a decrease in Ca(OH)2 was only seen when Biodentine™ exposed to acid.
The formation of Ca(OH)2 which influences the biological properties of calcium silicate cements was impaired by blood and acid exposures in ProRoot MTA, CEM Cement, and Retro MTA; however, in the case of Biodentine, only exposure to acid had this detrimental effect.
KeywordsBiodentine Calcium silicate cement CEM cement EDX MTA SEM XRD
The work was supported by Tehran University of Medical Sciences, Tehran, Iran (grant no. 27183).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
For this type of study, formal consent is not required.
- 5.Bakhtiar H, Nekoofar MH, Aminishakib P, Abedi F, Naghi Moosavi F, Esnaashari E, Azizi A, Esmailian S, Ellini MR, Mesgarzadeh V, Sezavar M, About I (2017) Human pulp responses to partial pulpotomy treatment with TheraCal as compared with Biodentine and ProRoot MTA: a clinical trial. J Endod 43:1786–1791. https://doi.org/10.1016/j.joen.2017.06.025 CrossRefGoogle Scholar
- 10.Esmaeili B, Alaghehmand H, Kordafshari T, Daryakenari G, Ehsani M, Bijani A (2016) Coronal discoloration induced by calcium-enriched mixture, mineral trioxide aggregate and calcium hydroxide: a spectrophotometric analysis. Iran Endod J 11(1):23–28. https://doi.org/10.7508/iej.2016.01.005 Google Scholar
- 11.Shokouhinejad N, Nekoofar MH, Pirmoazen S, Shamshiri AR, Dummer PM (2016) Evaluation and comparison of occurrence of tooth discoloration after the application of various calcium silicate-based cements: an ex vivo study. J Endod 42(1):140–144. https://doi.org/10.1016/j.joen.2015.08.034 CrossRefGoogle Scholar
- 17.Bakhtiar H, Mirzaei H, Bagheri MR, Fani N, Mashhadiabbas F, Baghaban Eslaminejad M, Sharifi D, Nekoofar MH, Dummer P (2017) Histologic tissue response to furcation perforation repair using mineral trioxide aggregate or dental pulp stem cells loaded onto treated dentin matrix or tricalcium phosphate. Clin Oral Investig 21:1579–1588. https://doi.org/10.1007/s00784-016-1967-0 CrossRefGoogle Scholar
- 29.Basturk FB, Nekoofar MH, Gunday M, Dummer PMH (2017) X-ray diffraction analysis of MTA mixed and placed with various techniques. Clin Oral Investig. https://doi.org/10.1007/s00784-017-2241-9
- 33.Bolhari B, Ashofteh Yazdi K, Sharifi F, Pirmoazen S (2015) Comparative scanning electron microscopic study of the marginal adaptation of four root-end filling materials in presence and absence of blood. J Dent (Tehran) 12(3):226–234Google Scholar
- 36.Grazziotin-Soares R, Nekoofar MH, Davies T, Hubler R, Meraji N, Dummer PMH (2017) Crystalline phases involved in the hydration of calcium silicate-based cements: semi-quantitative Rietveld X-ray diffraction analysis. Aust Endod J. https://doi.org/10.1111/aej.12226