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Clinical Oral Investigations

, Volume 22, Issue 7, pp 2511–2518 | Cite as

Lactobacillus rhamnosus intake can prevent the development of Candidiasis

  • Mariella Vieira Pereira Leão
  • Talita Angelina Aureliano Tavares
  • Célia Regina Gonçalves e Silva
  • Silvana Soleo Ferreira dos Santos
  • Juliana Campos Junqueira
  • Luciane Dias de Oliveira
  • Antonio Olavo Cardoso Jorge
Original Article

Abstract

Objective

This study aimed to investigate the influence of Lactobacillus rhamnosus intake on the development of candidiasis and cytokines release.

Material and methods

Candida suspensions were inoculated into the oral cavity of experimentally immunosuppressed mice for candidiasis induction. The animals were divided into experimental groups: candidiasis with no probiotic intake (F), candidiasis with probiotic intake during Candida inoculation (FP), and candidiasis with probiotic intake 14 days before inoculation with Candida (FPP); and control groups: (C), (CP), and (CPP) without inducing candidiasis with probiotic intake in the same manner as groups F, FP, and FPP, respectively. After these periods, samples were collected from the oral cavity for yeast counts and, after euthanasia, the tongues of the animals were removed for histological analysis. Sera samples were also collected for analysis of IL-1 beta, TNF-alpha, INF-gamma, IL-12, IL-4, and IL-10.

Results

FP group showed lower Candida counts in the oral cavity, and the presence of Candida was almost not detected in FPP group. In tissues, the counts of fungi were significantly lower in FPP group, followed by FP. Groups that consumed probiotics also had lower histological and inflammatory infiltrates compared to F. Cytokines analysis demonstrated low concentrations of TNF-α, IL-12, IL-4, and IL-10 in all the groups, and no statistical difference between them. The production of IL-6 could be better detected, and the experimental groups that consumed the probiotic showed significant lower levels of this cytokine.

Conclusions

The results suggest that L. rhamnosus intake, especially preventively, may avoid or decrease the development of candidiasis in immunosuppressed mice.

Clinical relevance

This work adds scientific evidences that probiotics intake can avoid the development of candidiasis.

Keywords

Probiotics Candida Lactobacillus rhamnosus Cytokine 

Notes

Funding

The work was supported by Fundação de Amparo à Pesquisa de São Paulo (FAPESP).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Informed consent

For this type of study, formal consent was not required.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Mariella Vieira Pereira Leão
    • 1
  • Talita Angelina Aureliano Tavares
    • 2
  • Célia Regina Gonçalves e Silva
    • 1
  • Silvana Soleo Ferreira dos Santos
    • 1
  • Juliana Campos Junqueira
    • 2
  • Luciane Dias de Oliveira
    • 2
  • Antonio Olavo Cardoso Jorge
    • 2
  1. 1.Bioscience Basic InstituteUniversity of TaubatéTaubatéBrazil
  2. 2.Department of Biosciences and Oral Diagnosis, Institute of Science and TechnologyUniv. Estadual Paulista, UNESPSão José dos CamposBrazil

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