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Clinical Oral Investigations

, Volume 22, Issue 5, pp 2069–2079 | Cite as

Therapeutic effect of andiroba oil (Carapa guianensis Aubl.) against oral mucositis: an experimental study in golden Syrian hamsters

  • Ana Márcia Viana Wanzeler
  • Sergio Melo Alves Júnior
  • Jessica Teixeira Gomes
  • Eduardo Henrique Herbster Gouveia
  • Higor Yuri Bezerra Henriques
  • Rosa Helena Chaves
  • Bruno Moreira Soares
  • Hugo Leonardo Crisóstomo Salgado
  • Alberdan Silva Santos
  • Fabrício Mesquita Tuji
Original Article

Abstract

Objectives

The aim of this study was to investigate the healing activity of andiroba (Carapa guianensis Aubl.) against oral mucositis (OM) induced by 5-fluorouracil in golden Syrian hamsters.

Materials and methods

A total of 122 animals were randomized and divided into six groups: andiroba oil 100%, andiroba oil 10%, andiroba oil 10% refined, no treatment group, all n = 28; and negative control (NC) and cyclophosphamide (CPA) groups, both n = 5. OM was induced by intraperitoneal administration of 60 mg/kg 5-FU on days 0, 5 and 10 followed by mechanical trauma on the oral mucosa on days 1 and 2. From day 1 to day 15, the animals of the andiroba group were treated three times a day. On days 4, 8, 12 and 15, the mucosa was photographed and removed for clinical and histopathological analysis. The bone marrow of the femur was removed and the micronucleus test was performed to evaluate the cytotoxicity and genotoxicity. The data were subjected to analysis of variance, followed by the Tukey and Bonferroni test.

Results

Treatment with 100% andiroba oil reduced the degree of OM compared to that reported in the other groups (p < 0.05). Andiroba oil at both concentrations was not cytotoxic, but treatment with 100% andiroba oil showed a genotoxic potential (p < 0.001).

Conclusions

Frequent administration of andiroba oil accelerated the healing process in an experimental model of 5-fluorouracil-induced OM. However, the genotoxicity of andiroba in other cell systems and under other conditions are being tested.

Clinical relevance

The use of andiroba in topical form may be associated with reduced intensity of OM. Seek therapeutic alternatives to minimize the pain and suffering that these side effects cause cancer patients is an important scientific step.

Keywords

Oral mucositis Cancer Oncology 5-fluorouracil Carapa guianensis Phytotherapy 

Notes

Acknowledgments

The authors thank the Experimental Research Group and the Laboratory of Histopathology of the University of the State of Pará (CESUPA) for the technical assistance provided by the Laboratory of Systematic Research in Biotechnology and Molecular Biodiversity of the Federal University of Pará (LabISisBio/UFPA) and by the Laboratory of Human Cytogenetics of the Federal University of Pará (LCH/UFPA).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies involving human participants performed by any of the authors. All procedures performed in studies with animals were in accordance with the ethical standards of the institutional and/or national research committee and all the care and use of animals were followed.

References

  1. 1.
    Cinausero M et al (2017) New frontiers in the pathobiology and treatment of cancer regimen-related mucosal injury. Front Pharmacol 8:354CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Keefe DM, Schubert MM, Elting LS, Sonis ST, Epstein JB, Raber-Durlacher JE, Migliorati CA, McGuire DB, Hutchins RD, Peterson D, for the Mucositis Study Section of the Multinational Association of Supportive Care in Cancer, and the International Society for Oral Oncology (2007) Updated clinical practice guidelines for the prevention and treatment of mucositis. Cancer 109(5):820–831.  https://doi.org/10.1002/cncr.22484 CrossRefPubMedGoogle Scholar
  3. 3.
    Sonis ST (2009) Mucositis: the impact, biology and therapeutic opportunities of oral mucositis. Oral Oncol 45(12):1015–1020.  https://doi.org/10.1016/j.oraloncology.2009.08.006 CrossRefPubMedGoogle Scholar
  4. 4.
    Sonis ST (2013) Oral mucositis in head and neck cancer: risk, biology, and management. Am Soc Clin Oncol Educ Book 33:e236–e240.  https://doi.org/10.1200/EdBook_AM.2013.33.e236 CrossRefGoogle Scholar
  5. 5.
    Lalla RV et al (2014) MASCC/ISOO clinical practice guidelines for the management of mucositis secondary to cancer therapy. Cancer 120(10):1453–1461CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Elad S, Meidan I, Sellam G, Simaan S, Zeevi I, Waldman E, Weintraub M, Revel-Vilk S (2013) Topical curcumin for the prevention of oral mucositis in pediatric patients: case series. Altern Ther Health Med 19(3):21–24PubMedGoogle Scholar
  7. 7.
    Hawley P, Hovan A, McGahan CE, Saunders DA (2014) Randomized palcebo-controlled trialof manuka honey for radiation-induced oral mucositis. Support Care Cancer 22(3):751–761.  https://doi.org/10.1007/s00520-013-2031-0 CrossRefPubMedGoogle Scholar
  8. 8.
    Mardani M, Afra SM, Tanideh N, Tadbir AA, Modarresi F, Koohi-Hosseinabadi O, Iraji A, Sepehrimanesh M (2016) Hydroalcoholic extract of Carum carvi L. in oral mucositis: a clinical trial in male golden hamsters. Oral Dis 22(1):39–46.  https://doi.org/10.1111/odi.12375 CrossRefPubMedGoogle Scholar
  9. 9.
    Tanideh N, Tavakoli P, Saghiri MA, Garciagodoy F, Amanat D, Tadbir AA, Samani SM, Tamadon A (2013) Healing acceleration in hamsters of oral mucositis induced by 5 -fluorouracil with topical Calendula officinalis. Oral Surg Oral Med Oral Pathol Oral Radiol 115(3):332–338.  https://doi.org/10.1016/j.oooo.2012.08.450 CrossRefPubMedGoogle Scholar
  10. 10.
    Yarom N, Ariyawardana A, Hovan A, Barasch A, Jarvis V et al (2013) Systematic review of natural agents for the management of oral mucositis in cancer patients. Support Care Cancer 21(11):3209–3221.  https://doi.org/10.1007/s00520-013-1869-5 CrossRefPubMedGoogle Scholar
  11. 11.
    Henriques MG, Penido C (2014) The therapeutic properties of Carapa guianensi. Curr Pharm Des 20(6):850–856.  https://doi.org/10.2174/13816128113199990048 CrossRefPubMedGoogle Scholar
  12. 12.
    Tappin MRR, Nakamura MJ, Siani AC, Lucchetti L (2008) Development of HPLC method for the determination of tetranortriterpenoids in Carapa guianensis seed oil by experimental design. J Pharm Biomed Anal 48(4):1090–1095.  https://doi.org/10.1016/j.jpba.2008.08.027 CrossRefPubMedGoogle Scholar
  13. 13.
    Penido C, Costa KA, Pennaforte RJ, Costa MF, Pereira JF et al (2005) Anti-allergiceffects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on allergeninduced vascular permeability and hyperalgesia. Inflamm Res 54(7):295–303.  https://doi.org/10.1007/s00011-005-1357-6 CrossRefPubMedGoogle Scholar
  14. 14.
    Penido C, Conte FP, Chagas MSS, Rodrigues CAB, Pereira JFG, Henriques MGMO (2006) Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice. Inflamm Res 55:57–464CrossRefGoogle Scholar
  15. 15.
    Nayak BS, Kanhai J, Milne DM, Pereira LP, Swanston WH (2011) Experimental evaluation of ethanolic extract of Carapa guianensis L. leaf for its wound healing activity using three wound models. Evid Based Complement Alternat Med 1:1–6Google Scholar
  16. 16.
    Costa-Silva JH, Lyra MMA, Lima CR, Arruda VM, Araújo AV et al (2006) Toxicological reproductive study of Carapa guianensis Aublet (andiroba) in female Wistar rats. Acta Farmacêutica Bonaerense 25:425–428Google Scholar
  17. 17.
    Costa-Silva JH, Lyra MMA, Lima CR, Arruda VM, Araújo AV, Ribeiro AR, Arruda AC, Fraga MCCA, Lafayette SSL, Wanderley AG (2007) A toxicological evaluation of the effect of carapa guianensis Aublet on pregnancy in Wistar rats. J Ethonopharmacol 112(1):122–126.  https://doi.org/10.1016/j.jep.2007.02.004 CrossRefGoogle Scholar
  18. 18.
    Costa-Silva JH, Lima CR, Silva EJ, Araújo AV, Fraga MC et al (2008) Acute and subacute toxicity of the Carapa guianensis Aublet (Meliaceae) seed oil. J Ethnopharmacol 116(3):495–500.  https://doi.org/10.1016/j.jep.2007.12.016 CrossRefPubMedGoogle Scholar
  19. 19.
    Miranda Júnior RNC, Dolabela MF, Silva MN, Póvoa MM, Maia JGS (2012) Antiplasmodial activity of the andiroba (Carapa guianensis Aubl., Meliaceae) oil and its limonoid-rich fraction. J Ethnopharmacol 142(3):679–683.  https://doi.org/10.1016/j.jep.2012.05.037 CrossRefPubMedGoogle Scholar
  20. 20.
    Sonis ST, Tracey C, Shklar G, Jenson J, Florine D (1990) An animal model for mucositis induced by cancer chemotherapy. Oral Surg Oral Med Oral Pathol Oral Radiol 69(4):437–443.  https://doi.org/10.1016/0030-4220(90)90376-4 CrossRefGoogle Scholar
  21. 21.
    Lima V, Brito GA, Cunha FQ, Rebouças CG, Falcão BA et al (2005) Effects of the tumor necrosis fator-alpha inhibitors pentoxifyline and thalidomide in short-term experimental oral mucositis in hamsters. Eur J Oral Sci 113(3):210–217.  https://doi.org/10.1111/j.1600-0722.2005.00216.x CrossRefPubMedGoogle Scholar
  22. 22.
    Ferraris FK, Rodrigues R, da Silva VP, Figueiredo R, Penido C, Henriques M (2011) Modulation of T lymphocyte and eosinophil functions in vitro by natural tetranortriterpenoids isolated from Carapa guianensis Aublet. Int Immunopharmacol 11(1):1–11.  https://doi.org/10.1016/j.intimp.2010.09.010 CrossRefPubMedGoogle Scholar
  23. 23.
    Barros FN, Farias MPO, Tavares JPC, Alves LC, Faustino MAG (2012) In vitro efficacy of oil from the seed of Carapa guianensis (andiroba) in the control of Felicola subrostratus. Brazilian. J Pharmacogn 22(5):1130–1133.  https://doi.org/10.1590/S0102-695X2012005000047 Google Scholar
  24. 24.
    CK S, Mehta V, Ravikumar L, Shah R, Pinto H et al (2004) Phase II doubleblind randomized study comparing oral aloe vera versus placebo to prevent radiation-related mucositis in patients with head-and-neck neoplasms. Int J Radiat Oncol Biol Phys 60:171–177CrossRefGoogle Scholar
  25. 25.
    Nayak BS, Kanhai J, Milne DM, Swanston WH, Mayers S, Eversley M, Rao AVC (2010) Investigation of the wound healing activity of Carapa guianensis L. (Meliaceae) bark extract in rats using excision, incision, and dead space wound models. J Med Food 13(5):1141–1146.  https://doi.org/10.1089/jmf.2009.0214 CrossRefPubMedGoogle Scholar
  26. 26.
    Arrebola DFA, Fernández LAR, Roche LD, Laurencio AA, Fernández YES, Novoa AV (2012) Genotoxic assessment of the Carapa guianensis Aublet seed oleaginous extract in Balb/c mice micronucleus assay. Revista de Toxicología en Línea 39:1–13Google Scholar
  27. 27.
    Fenech M, Kirsch-Volders M, Natarajan AT, Surralles J, Crott JW, Parry J, Norppa H, Eastmond D, Tucker JD, Thomas P (2011) Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells. Mutagenesis 26(1):125–132.  https://doi.org/10.1093/mutage/geq052 CrossRefPubMedGoogle Scholar
  28. 28.
    Ribeiro RL, Salvadori DMF, Marques EK (2003) Test of micronucleus in bone marrow of rodents in vivo. Environmental mutagenicity. Canoas: Editora Ulbra:173–200Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Ana Márcia Viana Wanzeler
    • 1
    • 2
  • Sergio Melo Alves Júnior
    • 1
  • Jessica Teixeira Gomes
    • 1
  • Eduardo Henrique Herbster Gouveia
    • 3
  • Higor Yuri Bezerra Henriques
    • 3
  • Rosa Helena Chaves
    • 3
  • Bruno Moreira Soares
    • 4
  • Hugo Leonardo Crisóstomo Salgado
    • 5
  • Alberdan Silva Santos
    • 5
  • Fabrício Mesquita Tuji
    • 1
  1. 1.Department of OdontologyFederal University of Pará, BelémBelémBrazil
  2. 2.BelémBrazil
  3. 3.Department of MedicineUniversity of the State of ParáBelémBrazil
  4. 4.Department of GeneticsHuman Cytogenetics Laboratory of the Federal University of ParáBelémBrazil
  5. 5.Department of ChemistrySystematic Research in Biotechnology and Molecular Diversity Laboratory of the Federal University of ParáBelémBrazil

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